| 从中国对虾分离纯化的一种杆状病毒及其超微结构的研究 |
| |
| 引用本文： | 石拓,孔杰,包振民,梁兴明,刘萍,王崇明.从中国对虾分离纯化的一种杆状病毒及其超微结构的研究[J].海洋学报,1998,20(2):60-64. |
| |
| 作者姓名： | 石拓 孔杰 包振民 梁兴明 刘萍 王崇明 |
| |
| 作者单位： | 1.中国水产科学研究院黄海水产研究所, 青岛266071 |
| |
| 基金项目： | 国家攀登计划B资助课题 |
| |
| 摘 要： | 从患病和濒死的中国对虾(Penaeus chinensis)头胸部分离提纯出一种杆状病毒,同时经对病虾的鳃、胃和中肠组织进行超薄切片及电镜观察,亦在上述组织的细胞核内发现大小相同、形态一致的大量杆状病毒粒子.成簇的病毒粒子主要在核内装配,并导致细胞结构的病变.病毒粒子大小约为320～400nm×100～130nm,两端钝圆,中部膨大,有囊膜,分内外两后,核衣壳大小为250～300nm×70～100nm.细胞内未观察到有核型多角体或颗粒体类包涵体存在.根据病毒学分类原则,该病毒应属于杆状病毒属无包涵体杆状病毒亚群,即杆状病毒C亚群.
|
| 关 键 词： | 中国对虾 非包涵体杆状病毒 分离提纯 电镜观察 |
| 收稿时间： | 1996/5/13 0:00:00 |
| 修稿时间： | 3/6/1997 12:00:00 AM |
Studies on Purification and ultrastructure of a baculoviral pathogen in Penaeus chinesis |
| |
| Shi Tuo,Kong Jie,Bao Zhenmin,Liang Xingming,Liu Ping and Wang Chongming.Studies on Purification and ultrastructure of a baculoviral pathogen in Penaeus chinesis[J].Acta Oceanologica Sinica (in Chinese),1998,20(2):60-64. |
| |
| Authors: | Shi Tuo Kong Jie Bao Zhenmin Liang Xingming Liu Ping and Wang Chongming |
| |
| Institution: | 1.Yellow Sea Fisheries Research Insititute, Qingdao 2660712.College of Marine Life Sciences, Ocean University of Qingdao, Qingdao, 266003 |
| |
| Abstract: | A kind of baculovirus was islated from the mid-gut gland tissues of sick of morbid Penaeus chinensis.Electron microscopic examination of ultrathin section of the gills, hepatopancrease and mid-gut gland tissues also revealed the presence of rod-shaped baculovirus particles with same sine in the affected cell nuclei.The virion covered with bilaminal envelope was 320-400 nm×100-130 nm in size,whereas the nucleocapsid ranged in the size of 250-300 nm in length and 70-100 nm in breadth respectively.No occlusion bodies have been found in cells.According to the principle of viral classification, this new found virus can probably belong to the non-occluded subgroup of insect baculoviridae,i.e.,C subgroup baculovirus. |
| |
| Keywords: | Penaeus chinensis non-occluded baculovirus isolation and purification electronic observation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《海洋学报》浏览原始摘要信息 |
| 点击此处可从《海洋学报》下载免费的PDF全文 |
|
