| 海藻工具酶研究Ⅱ.褐藻酸酶的制备、性质及对裙带菜细胞的解离研究 |
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| 引用本文： | 韩宝芹,戴继勋,刘万顺,王海.海藻工具酶研究Ⅱ.褐藻酸酶的制备、性质及对裙带菜细胞的解离研究[J].海洋学报,1998,20(2):90-95. |
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| 作者姓名： | 韩宝芹 戴继勋 刘万顺 王海 |
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| 作者单位： | 青岛海洋大学海洋生命学院, 青岛266003 |
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| 基金项目： | 国家科委资助，“八五”国家科技攻关计划生物技术项目 |
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| 摘 要： | 褐藻酸降解菌埃氏交替单胞菌(Alteromonas espejiana)菌株Al01,通过发酵培养制备褐藻酸酶.该酶作用的最适pH为7.0,最适温度为40℃,最适底物浓度为1%～2%;在离子浓度为0.5mmol/dm3时,Mn2+对酶促反应稍有促进作用,Ca2+、Hg2+则有明显的抑制作用.该酶用于裙带菜单细胞和原生质体解离时,以酶液组成为褐藻酸酶1%、纤维素酶1%,45×10-3的NaC1为渗透剂,酶解温度25℃,pH7.0,酶解3-4h效果最好.
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| 关 键 词： | 埃氏交替单胞菌 褐藻酸酶 裙带菜 原生质体 |
| 收稿时间： | 1996/5/10 0:00:00 |
| 修稿时间： | 6/3/1997 12:00:00 AM |
Studies on algal tool enzymes Ⅱ.Preparation and some properties of alginase and separation of cells in Undaria pinnatifida by enzymeHan Baoqin |
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| Han Baoyin,Dai Jixun,Liu Wanshun and Wang Hai.Studies on algal tool enzymes Ⅱ.Preparation and some properties of alginase and separation of cells in Undaria pinnatifida by enzymeHan Baoqin[J].Acta Oceanologica Sinica (in Chinese),1998,20(2):90-95. |
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| Authors: | Han Baoyin Dai Jixun Liu Wanshun and Wang Hai |
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| Institution: | Marine Life Sciences College, Ocean University of Qingdao, Qingdao 266003 |
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| Abstract: | Alginase was prepared by fermenting culture strain Al01 of alginate-degrading bacteria Alteromonas espejiana.The optimum temperature and pH for the enzyme were 40℃ and pH7,respectively.The optimum substratz concentration was 1%-2%.The enzyme activity was inhibited by Ca2+,Hg2+ and slightly activated by Mn2+ at ion concentration being 0.5 mmol/dm3.The optimum conditions of the separating mono-cells and protoplasts in Undaria pinnatifida were enzyme solution consisting of alginase 1%,cellulase 100, NaCl 45×10-3, pH 7.0 and incubation 3-4h at the 25℃. |
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| Keywords: | Alteromonas espejiana alginase Undaria pinnatifida protoplast |
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