| 斑纹鲟凝集素(CFL)的初步研究 Ⅱ.CFL的分子组成及性质鉴定 |
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| 引用本文： | 戴聪杰,王桂忠,李少菁,艾春香.斑纹鲟凝集素(CFL)的初步研究 Ⅱ.CFL的分子组成及性质鉴定[J].海洋学报,2006,28(3):98-102. |
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| 作者姓名： | 戴聪杰 王桂忠 李少菁 艾春香 |
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| 作者单位： | 1.厦门大学, 海洋学系, 福建, 厦门, 361005;厦门大学, 海洋环境科学教育部重点实验室, 福建, 厦门, 361005;泉州师范学院, 生物系, 福建, 泉州, 362000 |
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| 基金项目： | 福建省自然科学基金资助项目(B0410027)，泉州市科学技术局重点科技计划资助项目(Z200239)，国家863项目计划资助项目(2002AA603013) |
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| 摘 要： | 采用生化方法对斑纹鲟(Charybdis feriatus)凝集素进行了初步研究.结果表明,斑纹鲟的血清中有动物红细胞和微生物细胞凝集活性的凝集素(Charybdis feriatuslectin,CFL),其活力表现依赖于Ca2+,并为多种糖所抑制;CFL对40℃以上的温度敏感,其活力在pH6.0~8.0内稳定.经巯基乙醇处理和未经巯基乙醇处理的CFL在SDS-PAGE上均为一条蛋白带,说明斑纹鲟凝集素不含二硫键,且只有一种亚基,分子量为40.8 kDa,CFL氨基酸组成分析表明其富含酸性氨基酸.
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| 关 键 词： | 斑纹鲟凝集素(CFL) 糖抑制 热稳定性 分子量 氨基酸组成 |
| 文章编号： | 0253-4193（2006）03-0098-05 |
| 收稿时间： | 07 1 2005 12:00AM |
| 修稿时间： | 2005-07-012005-09-14 |
The preliminary studies of a lectin(CFL) from Charybdis feriatus Ⅱ.The molecular composition and the characterization of CFL |
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| DAI Cong-jie,WANG Gui-zhong,LI Shaojing and AI Chun-xiang.The preliminary studies of a lectin(CFL) from Charybdis feriatus Ⅱ.The molecular composition and the characterization of CFL[J].Acta Oceanologica Sinica (in Chinese),2006,28(3):98-102. |
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| Authors: | DAI Cong-jie WANG Gui-zhong LI Shaojing and AI Chun-xiang |
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| Institution: | 1.Department of Oceanography, Xiamen University, Xiamen 361005, China;Key Lab oratory Marine Environmental Sciences of Ministry of Education, Xiamen University, Xiamen 361005, China;Department of Biology, Quanzhou Normal College, Quanzhou 362000, China2.Department of Oceanography, Xiamen University, Xiamen 361005, China;Key Lab oratory Marine Environmental Sciences of Ministry of Education, Xiamen University, Xiamen 361005, China |
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| Abstract: | Lectin of Charybdis feriatus(shortly call CFL)was studied by biochemistry methods.The results show that the lectin agglutinates every kinds RBC from different animals and microbial cells tested widely in the experiment,as a Ca2+ dependent lect in CFL is inhibited by various sugars.CFL is sensitive to the temperature over 40 e and to the pH over 9.0 or below 5.0.No matter adding or not adding-S-S-reducant to the sample of CFL,it demonstrated one single band on plate SDS-PAGE.It shows that CFL does not contain-S-S-,and its molecular mass is 40.8kDa.The analysis result of aminoacid composition of CFL indicates that acidity amino acids were abundant. |
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| Keywords: | CFL(lectin from crab Charybdis feriatus) sugar inhibition thermostability molecula weight amino acid composition |
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