| 印度洋深海沉积物样品纯培养细菌的分离鉴定 |
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| 引用本文： | 姜钊,张卫花.印度洋深海沉积物样品纯培养细菌的分离鉴定[J].海洋学报,2022,44(4):104-113. |
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| 作者姓名： | 姜钊 张卫花 |
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| 作者单位： | 1.西藏民族大学 藏药检测技术教育部工程研究中心，陕西 咸阳 712082 |
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| 基金项目： | 西藏自治区自然科学基金项目;西藏自治区科技创新基地自主研究项目;西藏民族大学青年学人培育计划 |
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| 摘 要： | 为探索印度洋深海沉积物中纯培养细菌的多样性，本文对采自印度洋12个沉积物样品进行细菌纯培养分离，共获得343株细菌。所有细菌采用16S rRNA 基因进行比对分析，鉴定为4个门:厚壁菌门（Firmicutes）、变形菌门（Proteobacteria）、放线菌门（Actinobacteria）和拟杆菌门（Bacteroidetes），5个纲，13个目，26个科，39个属下的121个种。分离出的优势类群为链霉菌属（Streptomyces，分离率为16.53%）和芽孢杆菌属（Bacillus，分离率为8.26%），其中可能有12个潜在的新分类单元。研究结果表明，印度洋深海沉积环境中纯培养细菌资源丰富，潜在新物种较多；其次，分离培养方法与从海洋沉积物中获得的细菌物种多样性直接相关。
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| 关 键 词： | 印度洋 沉积环境 细菌 分离鉴定 |
| 收稿时间： | 2021-08-16 |
Isolation and identification of cultivable bacteria isolated from deep-sea sediment samples of the Indian Ocean |
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| Jiang Zhao,Zhang Weihua.Isolation and identification of cultivable bacteria isolated from deep-sea sediment samples of the Indian Ocean[J].Acta Oceanologica Sinica (in Chinese),2022,44(4):104-113. |
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| Authors: | Jiang Zhao Zhang Weihua |
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| Institution: | 1.Engineering Research Center of Tibetan Medicine Detection Technology, Ministry of Education, Xizang Minzu University, Xianyang 712082, China2.Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, Xizang Minzu University, Xianyang 712082, China |
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| Abstract: | In order to explore the diversity of pure cultured bacteria in the deep-sea sedimentary environment of the Indian Ocean, bacteria were isolated and identified from 12 sediment samples collected from the Indian Ocean. A total of 343 strains of bacteria were obtained by pure culture isolation method and 16S rRNA gene sequence analysis showed that these isolates belonged to Firmicutes, Proteobacteria, Actinobacteria, Bacteroidete and were divided into 5 classes, 13 orders, 26 families and 121 species under 39 genera. The dominant taxa were Streptomyces (separation rate is 16.53%) and Bacillus (separation rate is 8.26%), among which there were 12 potential new taxa. The results show that the deep-sea sedimentary environment of the Indian Ocean is rich in culturable bacteria and contains many new species; secondly, the isolation and culture methods are directly related to the bacterial species diversity obtained from marine sediments. |
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