| 海假交替单胞菌(Pseudoalteromonas marina)pilZ基因缺失抑制厚壳贻贝附着变态 |
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| 引用本文： | 张驰,王劲松,杨金龙,张俊波,万荣,梁箫.海假交替单胞菌(Pseudoalteromonas marina)pilZ基因缺失抑制厚壳贻贝附着变态[J].海洋学报,2022,44(4):95-103. |
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| 作者姓名： | 张驰 王劲松 杨金龙 张俊波 万荣 梁箫 |
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| 作者单位： | 1.上海海洋大学 国家海洋生物科学国际联合研究中心，上海 201306 |
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| 基金项目： | 国家自然科学基金（41876159,41476131）；;国家重点研发计划（2019YFC0312104,2020YFD0900804）； |
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| 摘 要： | 为探究海假交替单胞菌pilZ基因的缺失对生物被膜形成及突变菌生物被膜对厚壳贻贝幼虫附着变态的影响,本文通过同源重组构建pilZ基因缺失菌,分析了基因缺失菌生物被膜的细菌密度、膜厚、c-di-GMP水平和胞外产物含量等特性的变化及其对厚壳贻贝幼虫附着变态的调控作用.结果表明:与野生型菌株相比,pilZ基因缺失菌形成的生物...
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| 关 键 词： | pilZ 生物被膜 厚壳贻贝 附着变态 胞外产物 |
| 收稿时间： | 2021-11-10 |
Knockout of Pseudoalteromonas marina pilZ gene inhibited the settlement and metamorphosis of Mytilus coruscus |
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| Zhang Chi,Wang Jinsong,Yang Jinlong,Zhang Junbo,Wan Rong,Liang Xiao.Knockout of Pseudoalteromonas marina pilZ gene inhibited the settlement and metamorphosis of Mytilus coruscus[J].Acta Oceanologica Sinica (in Chinese),2022,44(4):95-103. |
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| Authors: | Zhang Chi Wang Jinsong Yang Jinlong Zhang Junbo Wan Rong Liang Xiao |
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| Abstract: | To investigate the effect of Pseudoalteromonas marina pilZ gene knockout on the biofilm formation and its influence on the settlement and metamorphosis of Mytilus coruscus larvae, ΔpilZ was constructed by homologous recombination, and the changes in bacterial density, biofilm thickness, c-di-GMP level and extracellular polymeric substances (EPS) of ΔpilZ bacteria biofilm were analyzed, as well as the regulation of the settlement and metamorphosis of M. coruscus larvae. The results showed that compared with the wild-type strain, the biofilm formed by ΔpilZ strain significantly increased the biofilm thickness, the number of bacteria, while the contents of β-polysaccharides and proteins in EPS were decreased and the settlement and metamorphosis of M. coruscus larvae were inhibited (p<0.05). There was no significant difference in c-di-GMP level, α-polysaccharide and lipid contents (p>0.05). In conclusion, P. marina pilZ gene knockout can regulate the bacterial biofilm and the content of EPS including β-polysaccharides and proteins contents, then inhibit the settlement and metamorphosis of M. coruscus larvae. |
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