| 中国对虾黄、渤海沿岸地理群的RAPD分析 |
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| 引用本文： | 刘萍,孔杰,石拓,庄志猛,邓景耀,徐怀恕,韩玲玲.中国对虾黄、渤海沿岸地理群的RAPD分析[J].海洋学报,2000,22(5):88-93. |
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| 作者姓名： | 刘萍 孔杰 石拓 庄志猛 邓景耀 徐怀恕 韩玲玲 |
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| 作者单位： | 1.中国水产科学研究院黄海水产研究所, 青岛 266071 |
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| 基金项目： | 国家自然科学基金重点资助项目（编号：39620260） |
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| 摘 要： | 采用随机扩增多态DNA(random amplified polymorphic DNA,简称RAPD)技术,对中国对虾的黄、渤海地理群的32个个体遗传多样性进行了检测,使用OPI系列的20个10bp的寡核苷酸随机引物,对每个个体的基因组DNA进行扩增,结果为:17个引物获得了谱带清晰且重复性好的产物,扩增片段的分子量在2000～2000bp之间,共检测106个位点,其中多态位点数为39个,占36.8%;平均遗传距离为0.0941+0.0206,有67个标记(占63.2%)在整个群体的32个个体间表观稳定的一致性.
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| 关 键 词： | 中国对虾 黄海、渤海种群 RAPD技术 遗传多样性 |
| 收稿时间： | 2/1/1999 12:00:00 AM |
| 修稿时间： | 1999-02-01 |
RAPD analysis of wild stock of penaeid shrimp (Penaeus chinensis ) in the China's coastal waters of Huanghai and Bohai Seas |
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| Liu Ping,Kong Jie,Shi Tuo,Zhuang Zhimeng,Deng Jingyao,Xu Huaishu and Han Lingling.RAPD analysis of wild stock of penaeid shrimp (Penaeus chinensis ) in the China''s coastal waters of Huanghai and Bohai Seas[J].Acta Oceanologica Sinica (in Chinese),2000,22(5):88-93. |
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| Authors: | Liu Ping Kong Jie Shi Tuo Zhuang Zhimeng Deng Jingyao Xu Huaishu and Han Lingling |
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| Institution: | 1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 2660712.Ocean University of Qingdao, Qingdao 266003 |
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| Abstract: | Genetic diversities of 32 individuals of P.Chinensis in the China's coastal waters of the Huanghai and Bohai Seas are detected by RAPD technique.20 decamer primers of OPI are used for DNA amplification for each individual.The results show that of reproducible fingerprints 17 primers are obtained,and the bands are clear.39 of 106 loci detected are polymorphic,amounting to 36.8%.Mean genetic distance is 0.094±0.0206; 68 markers (63.2% of the total) show stable homogeneity in all of the 32 individuals. |
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| Keywords: | Penaeus chinensis stock of the Huanghai and Bohai Seas RAPD technique genetic diversity |
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