| 海洋生境芽孢杆菌(Bacillus spp.)的培养条件及产生的胞外抗菌蛋白 |
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| 引用本文： | 田黎,李光友.海洋生境芽孢杆菌(Bacillus spp.)的培养条件及产生的胞外抗菌蛋白[J].海洋学报,2001,23(4):87-92. |
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| 作者姓名： | 田黎 李光友 |
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| 作者单位： | 国家海洋局第一海洋研究所, 山东青岛266061 |
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| 基金项目： | 国家海洋局重点实验室基金资助项目(98-001);山东省科技深海重点资助项目(601164604). |
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| 摘 要： | 测试了4株海洋芽孢杆菌的培养条件对其产生抗菌蛋白的影响;提取的粗蛋白的性质以及对6种病原指示菌的抑菌活性,结果表明,4株海洋芽孢杆菌在2216E,EMB,CM培养液;15～35℃;NaCl浓度0%～5%;pH3～9的条件下均能生长,适宜生长温度20～28℃;NaCl浓度1%～3%;pH5～8.菌株分别培养84～108h抑菌活性最强.(NH4)2SO4沉淀抗菌粗蛋白的最适饱和度为50%～70%.粗蛋白对病原菌尤其对病原真菌具有强烈的抑制作用.粗蛋白性质稳定,对高温和蛋白酶不敏感.
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| 关 键 词： | 海洋生境 芽孢杆菌 培养条件 抗菌蛋白 |
| 文章编号： | 0253-4193(2001)04-0087-06 |
| 收稿时间： | 1/4/2000 12:00:00 AM |
| 修稿时间： | 2000年1月4日 |
Cultural conditions of marine Bacillus spp. and effect on produced antagonistic protein |
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| TIAN Li and LI Guang-you.Cultural conditions of marine Bacillus spp. and effect on produced antagonistic protein[J].Acta Oceanologica Sinica (in Chinese),2001,23(4):87-92. |
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| Authors: | TIAN Li and LI Guang-you |
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| Institution: | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China |
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| Abstract: | The antagonistic protein production, which inhibits 6 species fungi and bacteria from marine Bacillus spp., and the cultural conditions such as temperature, fermentation peried, NaCl concentration and pH of 4 strians marine bacter ia are studied.The result of test shows that suittable factor for both bacteria growth and antimicrobial substance produce are 20~28, 84~108 h, NaCl 1%~3%, pH5~8.Concentration of ammonium sulfate between 50% and 70% is the most fitting saturation for antagonistic protein precipitability.The proteins are insensitive to heat and protein and have high inhibitory value to pathogenetic fungi. |
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| Keywords: | marine Bacillus spp cultural conditions antagonistic protein |
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