| 低氧胁迫对军曹鱼幼鱼生长、血清生化和非特异性免疫指标的影响 |
| |
| 引用本文： | 王维政,曾泽乾,黄建盛,郭志雄,李洪娟,陈刚.低氧胁迫对军曹鱼幼鱼生长、血清生化和非特异性免疫指标的影响[J].海洋学报,2021,43(2):49-58. |
| |
| 作者姓名： | 王维政 曾泽乾 黄建盛 郭志雄 李洪娟 陈刚 |
| |
| 作者单位： | 1.广东海洋大学 水产学院，广东 湛江 524088 |
| |
| 基金项目： | 现代农业产业技术体系专项资金(CARS-47);南方海洋科学与工程广东省实验室(湛江)资助项目(ZJW-2019-06);广东海洋大学大学生创新创业训练计划项目(CXXL2018131)。 |
| |
| 摘 要： | 为了研究低氧胁迫对军曹鱼幼鱼生长、血清生化和非特异性免疫指标的影响，将体重为(50.44±2.78) g的军曹鱼幼鱼在(3.15±0.21) mg/L的低氧环境下养殖4周，分别在第1天、第7天、第14天、第28天取样并进行相关指标的测定。结果表明，在低氧胁迫过程中:(1)军曹鱼幼鱼生长受到低氧的抑制，增重率和特定生长率显著低于对照水平(p<0.05)。(2)血清肝功能指标表现为不同程度的上升趋势，其中谷草转氨酶(AST)在第7天、第14天、第28天极显著高于对照组(p<0.01)；谷丙转氨酶活性在第14天、第28天极显著高于对照组(p<0.01)；总蛋白和白蛋白含量均在第14天和第28天与对照组有显著性差异(p<0.05)。(3)血清糖脂水平表现为波动升高的趋势，其中血糖含量在第1天、第14天和第28天与对照水平有显著性差异(p<0.05)；甘油三酯含量在第14天和第28天极显著高于对照水平(p<0.01)；总胆固醇含量在第1天显著下降(p<0.05)后呈不断上升的趋势。(4)血清离子含量变化情况各异，其中钠离子和氯离子含量在第14天和第28天极显著升高(p<0.01)；钾离子含量不断升高并在第7天、第14天和第28天与对照组具有显著性差异(p<0.05)；钙离子含量则呈不断下降的变化趋势，在第14天和第28天显著低于对照水平(p<0.05)。(5)血清超氧化物歧化酶活性持续升高，并在第7天、第14天、第28天极显著高于对照组(p<0.01)；丙二醛含量持续上升，并在第14天、第28天极显著高于对照组(p<0.01)；过氧化氢酶活性表现为“下降?上升?下降”变化趋势；溶菌酶活性在第1天显著升高(p<0.05)后下降。研究结果显示，低氧胁迫对军曹鱼幼鱼造成一定程度的氧化损伤，抑制其生长并使血清生化和非特异性免疫相关指标发生显著变化，表明军曹鱼机体物质代谢和免疫系统受到影响。
|
| 关 键 词： | 军曹鱼 低氧 生长 生化指标 非特异性免疫 |
| 收稿时间： | 2020-05-02 |
Hypoxia stress on growth,serum biochemical and non-specific immune indexes of juvenile cobia (Rachycentron canadum) |
| |
| Wang Weizheng,Zeng Zeqian,Huang Jiansheng,Guo Zhixiong,Li Hongjuan,Chen Gang.Hypoxia stress on growth,serum biochemical and non-specific immune indexes of juvenile cobia (Rachycentron canadum)[J].Acta Oceanologica Sinica (in Chinese),2021,43(2):49-58. |
| |
| Authors: | Wang Weizheng Zeng Zeqian Huang Jiansheng Guo Zhixiong Li Hongjuan Chen Gang |
| |
| Institution: | 1.Fishery College, Guangdong Ocean University, Zhanjiang 524088, China2.Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, China3.Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China |
| |
| Abstract: | In order to study the effect of hypoxia stress on serum of biochemical and non-specific immune indexes of the juvenile cobia(Rachycentron canadum),the fish with body weight of(50.44±2.78)g were treated at dissolved oxygen content of(3.15±0.21)mg/L for 4 weeks.The samples were taken on 1 d,7 d,14 d and 28 d respectively for determination of relevant indexes and results showed that:(1)the growth of juvenile cobia was inhibited by hypoxia,the weight gain rate and specific growth rate were significantly lower than the control level(p<0.05);(2)the indexes of liver function showed that activities of aspartate aminotransferase and alanine aminotransferase significantly increased(p<0.01)on 7 d,14 d,28 d and 14 d,28 d respectively,while the content of total protein and albumin significantly increased(p<0.05)on 14 d and 28 d than that of the control group;(3)the content of blood glucose significantly increased(p<0.05)on 1 d,14 d and 28 d,while the triglyceride significantly increased(p<0.01)on 14 d and 28 d.The total cholesterol content significantly decreased(p<0.05)on 1 d,then continue to increase;(4)the serum ion content changed with different trend,in which sodium ion and chloride ion significantly increased(p<0.01)on 14 d and 28 d.Potassium content increased continuously and was significantly higher(p<0.05)on 7 d,14 d and 28 d than control level,while calcium content continued to decrease and there was a significant difference(p<0.05)on 14 d and 28 d;(5)activitie of superoxide dismutase and content of malondialdehyde continued to increase and were significantly higher(p<0.01)than those in the control group on 7 d,14 d,28 d and 14 d,28 d respectively.Activities of catalase showed significantly“decreased-increased-decreased”patterns.Activitie of lysozyme significantly increased(p<0.05)on 1 d and then continued to decrease.These results showed that hypoxia stress caused oxidative damage to the juvenile cobia,inhibited its growth and led to significant change in serum biochemical and non-specific immune indexes,which indicating that the nutrient utilization and immune system of cobia were affected. |
| |
| Keywords: | Rachycentron canadum hypoxia growth biochemical indexes non-specific immune |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《海洋学报》浏览原始摘要信息 |
| 点击此处可从《海洋学报》下载免费的PDF全文 |
|
