| RSM方法用于产红色素发酵培养基优化 |
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| 引用本文： | 武洪庆,田黎,张久明,温占波.RSM方法用于产红色素发酵培养基优化[J].海洋学报,2005,27(3):142-146. |
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| 作者姓名： | 武洪庆 田黎 张久明 温占波 |
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| 作者单位： | 1.国家海洋局, 海洋生物活性物质重点实验室, 山东, 青岛, 266061;河北工业大学, 天津, 300130 |
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| 基金项目： | 国家“863”资助项目(2001AA620402)，国家海洋局科技兴海资助项目(2000112)，青岛市科技重点资助项目(JXM0111). |
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| 摘 要： | 用RSM方法对黄杆菌属产红色素菌株S-9801(Flavobacterium sp.)的培养基进行了优化.首先用部分重复因子实验对培养基组分蛋白胨、酵母粉、葡萄糖及NaCl浓度对菌株产红色素的影响进行评价,找出主要影响因子为葡萄糖和NaCl浓度.两者均为正影响,其他组分对色素含量的影响不显著.第二步用最陡爬坡路径逼近最大响应区域.最后用中心组合设计及响应面分析确定主要影响因子的最佳浓度.菌株在优化培养基中培养较初始培养基色素含量提高320%.
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| 关 键 词： | RSM方法 培养基优化 色素 |
| 文章编号： | 0253-4193(2005)03-0142-05 |
| 收稿时间： | 2003/3/18 0:00:00 |
| 修稿时间： | 6/9/2003 12:00:00 AM |
Culture medium optimization for pigment production with RSM method |
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| WU Hong-qing,TIAN Li,ZHANG Jiu-ming and WEN Zhan-bo.Culture medium optimization for pigment production with RSM method[J].Acta Oceanologica Sinica (in Chinese),2005,27(3):142-146. |
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| Authors: | WU Hong-qing TIAN Li ZHANG Jiu-ming and WEN Zhan-bo |
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| Institution: | 1.Ley Lab oratory of Marine Biological Active Substance, First Institute of Oceanography, Qingdao 266061, China;Hebei Univerity of Technology, Tianjin 300130, China2.Ley Lab oratory of Marine Biological Active Substance, First Institute of Oceanography, Qingdao 266061, China;Qindao University of Science and Technology, qindao 266042, Chian3.Ley Lab oratory of Marine Biological Active Substance, First Institute of Oceanography, Qingdao 266061, China |
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| Abstract: | Response surface methodology was used to optimize a medium for pigment production of marine bacterium S-9801 strain. In the first optimization step the influence of yeast extract,peptone, glucose and sodium chloride on pigment production was evaluated using a fractional factorial design.Glucose and sodium chloride influenced pigment production positively while other components had no significant effect. The path of steepest ascent was used to approach the optimal region of the medium composition. In the third step the optional concentration of glucose and sodium chloride was determined by a central composite design and response surface analysis.The optimized medium allowed pigment production(OD_(535~650))to be increased from 463 to 559, 320% higher than the original medium. |
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| Keywords: | response surface methodology red pigment optimization |
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