| 用于紫菜无性系种质鉴定的计算机化DNA指纹的建立 |
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| 引用本文： | 贾建航,陈一华,石金锋,金德敏,许璞,梅俊学,翁曼丽,王斌.用于紫菜无性系种质鉴定的计算机化DNA指纹的建立[J].海洋学报,2001,23(1):79-84. |
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| 作者姓名： | 贾建航 陈一华 石金锋 金德敏 许璞 梅俊学 翁曼丽 王斌 |
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| 作者单位： | 1.中国科学院遗传研究所, 北京 100101 |
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| 基金项目： | 国家“863”计划海洋领域资助项目(819-03-02). |
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| 摘 要： | 研究用RAPD技术对条斑紫菜(Porphyra yezoensis)、坛紫菜(P.Haitanensis)、半叶紫菜(P.Katadai var.Hemiphylla)和少精紫菜(P.Oligospermatangia)的15个无性系丝状体进行了遗传多样分析,用120个Operon引物进行了筛选,从中选用来自OPJ-18和OPN-02扩增出的8条多态性条带构建了这15个紫菜无性系的DNA指纹图谱,在该图谱中每个紫菜无性系均有各自特异的DNA指纹.用1和0分别表示DNA带的出现和缺失,将各个无性系的DNA指纹用计算机语言表示,建立了15个紫菜无性系的计算机化DNA指纹;在此基础上设计了紫菜无性系种质鉴定的计算机软件PhGI.
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| 关 键 词： | 计算机化的DNA指纹 种质鉴定 RAPD 紫菜 |
| 文章编号： | 0253-4193(2001)01-0079-06 |
| 收稿时间： | 6/2/2000 12:00:00 AM |
| 修稿时间： | 2000年6月2日 |
Construction of computerized DNA fingerprinting for identification of Porphyra lines |
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| JIA Jian-hang,CHEN Yi-hu,SHI Jin-feng,JIN De-min,XU Pu,MEI Jun-cue,WENG Man-li and WANG Bin.Construction of computerized DNA fingerprinting for identification of Porphyra lines[J].Acta Oceanologica Sinica (in Chinese),2001,23(1):79-84. |
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| Authors: | JIA Jian-hang CHEN Yi-hu SHI Jin-feng JIN De-min XU Pu MEI Jun-cue WENG Man-li and WANG Bin |
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| Institution: | 1.Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China2.Jiangsu Institute of Oceanology and Hydrobiology, Nantong 226007, China3.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China |
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| Abstract: | RAPD (randomly amplified polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing P. Yezoensis, P. Haitanensis, P. Katadai var. hemiphylla and P. oligospermatangia. Eight stable and repeatable RAPD bands amplified with two primers, OPN - 02 and OPJ - 18, were selected for the construction of DNA fingerprinting. The RAPD results are scored based on the presence or absence of each of the 8 bands and then converted to computer language expressed with two digitals, 1 and 0, which represent the presence (numbered as l) or absence (numbered as 0) of each band, respectively. Baed on these results, a model DNA fingerprint and a computerized DNA fingerprint are constructed. In the constructed DNA fingerprint, each Porline has its unique fingerprinting pattern and can be easily distinguished from each other. Later, a software, named as PhGI, is designed bed on this DNA fingerprinting for Porphyra line identification. |
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| Keywords: | computerized DNA fingerprinting germplasm identification RAPD Porphyra |
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