| 鲸类线粒体基因组特征分析及分子标记探讨 |
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| 引用本文： | 田美,申欣,孟学平,程汉良.鲸类线粒体基因组特征分析及分子标记探讨[J].海洋学报,2011,33(5):104-114. |
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| 作者姓名： | 田美 申欣 孟学平 程汉良 |
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| 作者单位： | 淮海工学院 海洋学院,江苏 连云港 222005 |
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| 基金项目： | 国家自然科学基金(40906067);江苏省海洋生物技术重点建设实验室基金(2009HS13);淮海工学院自然科学基金(Z2009048);江苏省"青蓝工程"人才基金。 |
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| 摘 要： | 综合分析鲸类32个物种的线粒体基因组全序列,全面揭示了鲸类线粒体基因组的基本特征、蛋白质编码基因、选择压力和差异位点等.鲸类线粒体基因组均编码后生动物标准的37个基因.绝大多数鲸类线粒体基因组的基因排列顺序完全相同,而且与脊椎动物线粒体基因组的典型排列一致.4个类群(真露脊鲸属、须鲸属、原海豚属和宽吻海豚属)线粒体基因...
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| 关 键 词： | 鲸目 线粒体基因组 差异位点 分子标记 |
| 收稿时间： | 2010/5/18 0:00:00 |
Analysis of the characteristics of whales mitochondrial genomes and exploration of molecular markers |
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| TIAN Mei,SHEN Xin,MENG Xue-ping and CHENG Han-liang.Analysis of the characteristics of whales mitochondrial genomes and exploration of molecular markers[J].Acta Oceanologica Sinica (in Chinese),2011,33(5):104-114. |
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| Authors: | TIAN Mei SHEN Xin MENG Xue-ping and CHENG Han-liang |
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| Institution: | College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005,China |
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| Abstract: | The basic characteristics (including protein coding genes, selection pressure and different loci etc.) of whales mitochondrial genomes were fully revealed by comprehensive analysis of 32 whales mitochondrial genomes. Whales mitochondrial genomes contain 37 standard metazoan genes and their gene order is identical.. The Ka/Ks ratio of the whales (Eubalaena, Balaenoptera, Stenella and Tursiops) 13 mitochondrial protein coding genes is lower than 1 (range between 0 and 0.587 7), indicating for the purifying selection (negative selection). The average Ka/Ks of cox3 gene is the lowest, followed by cox1 , cox2 and cob genes, indicating that these genes bear a strong selection pressure and functional constraints. Meanwhile, the average Ka/Ks of nad6 gene is the highest, followed by atp8 , nad2 and atp6 gene, indicating these genes bear weaker selection pressure. The genetic variation analysis of main genes (13 protein coding genes and 2 ribosomal RNA genes) and D-loop region within 32 species and intra-genus (Eubalaena, Balaenoptera, Stenella and Tursiops) showed that nad5 , nad4 and nad2 gene are ideal molecular markers and can be used as supplementary molecular markers to cox1 gene, which providing a reference for the conservation of whales biological diversity and use of the biological resources rationally. |
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| Keywords: | Cetacea mitochondrial genome genetic different loci molecular marker |
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