| 基于线粒体DNA部分片段探讨条石鲷与斑石鲷的亲缘关系 |
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| 引用本文： | 肖志忠,肖永双,马道远,徐世宏,刘清华,李军.基于线粒体DNA部分片段探讨条石鲷与斑石鲷的亲缘关系[J].海洋学报,2011,33(5):115-123. |
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| 作者姓名： | 肖志忠 肖永双 马道远 徐世宏 刘清华 李军 |
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| 作者单位： | 中国科学院 海洋研究所 海洋生物技术工程研究发展中心,山东 青岛 266071 |
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| 基金项目： | 国家农业科技成果转化资金项目(05EFN2166000453);中国科学院创新前沿项目(KSCX2-YW-N-47-08)。 |
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| 摘 要： | 比较分析了条石鲷和斑石鲷线粒体基因组COI、Cyt b基因以及D-loop片段总长度为1 948 bp的核苷酸序列,探讨了条石鲷和斑石鲷的遗传分化程度.两种间共检测到314个多态位点,蛋白质编码基因上的核苷酸替代主要是发生在密码子第3位点上的同义替换.核苷酸组成分析结果表明:三个目的片段的鸟嘌呤(G)含量普遍较低,在两...
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| 关 键 词： | 条石鲷 斑石鲷 线粒体DNA 遗传距离 分化时间 |
| 收稿时间： | 2010/12/21 0:00:00 |
Relationship between Oplegnathus fasciatus and O. punctatus revealed by mtDNA sequence characteristics |
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| XIAO Zhi-zhong,XIAO Yong-shuang,MA Dao-yuan,XU Shi-hong,LIU Qing-hua and LI jun.Relationship between Oplegnathus fasciatus and O. punctatus revealed by mtDNA sequence characteristics[J].Acta Oceanologica Sinica (in Chinese),2011,33(5):115-123. |
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| Authors: | XIAO Zhi-zhong XIAO Yong-shuang MA Dao-yuan XU Shi-hong LIU Qing-hua and LI jun |
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| Institution: | Marine Biotech R & D Center,Institute of Oceanology, Chinese Academy of Science, Qingdao 266071,China |
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| Abstract: | Oplegnathus fasciatus and O. punctatus, belonging to the family Oplegnathidae, are commercially important fisheries resources in China. To date, no researches have been carried out on the relationship between O. fasciatus and O. punctatus by mitochondrial DNA sequences characteristics. Partial nucleotide sequences of mitochondrial DNA COI gene, Cyt b gene and D-loop fragment were sequenced and analyzed to estimate the degree of genetic differentiation between O. fasciatus and O. punctatus. One hundred and three nucleotide substitutions were checked in the total 1 107 bp sequences analyzed, and most of them were synonymous transitions at the third codon positions in the two protein-coding genes. Nucleotide composition analysis indicated a strong bias against guanine (G) in the three fragments, especially in the third codon positions of COI and Cyt b genes. The mean genetic distances based on the COI, Cyt b and D-loop fragments between O. fasciatus and O. punctatus were 0.097, 0.080 and 0.172, respectively. The average genetic distances showed interspecific differentiation within genus. There were obvious differences in the rates of nucleotide substitution between the two species in the three mtDNA segments. In contrast with previous research, the rate of nucleotide substitution for COI gene fragment was higher than that of Cyt b fragment in the present study. The estimated divergence time between O. fasciatus and O. punctatus based on Cyt b and ND5 genes was about 3.85~5.3 million years ago, which indicated a Early Pliocene divergence of the two species. |
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| Keywords: | Oplegnathus fasciatus O punctatus mitochondrial DNA genetic distances divergence time |
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