首页 | 本学科首页   官方微博 | 高级检索  
     检索      

栉孔扇贝第1卵裂抑制型雌核发育二倍体的研究
引用本文:潘英,李琪,于瑞海,王如才.栉孔扇贝第1卵裂抑制型雌核发育二倍体的研究[J].海洋学报,2005,27(6):154-161.
作者姓名:潘英  李琪  于瑞海  王如才
作者单位:1.中国海洋大学水产学院 山东青岛 266003;广西大学动物科技学院 广西南宁 530005
基金项目:国家自然科学基金资助项目(30170735);教育部重点资助项目(104114)
摘    要:利用6-二甲基氨基嘌呤(60μg/cm3;6-DMAP)和细胞松弛素B(0.5μg/cm3;CB)抑制受精卵的第1次卵裂,授精后60和65 min分别用CB和6-DMAP持续处理20和15 min,可诱导出12.5%和27.5%的第1卵裂抑制型雌核发育二倍体.细胞学观察显示,6-DMAP抑制第1次卵裂产生的雌核发育二倍体主要在第1次有丝分裂后期的受精卵,由于破坏了纺锤体和阻止了染色体分离,导致一个二倍性雌核的形成,而CB有效地阻止了第1次卵裂的胞质分裂.尽管倍化率和D型幼虫发生率较低,但该研究首次报道了栉孔扇贝第1卵裂抑制型雌核发育二倍体诱导的可行性.

关 键 词:栉孔扇贝    雌核发育二倍体    6-二甲基氨基嘌呤    细胞松弛素B    细胞学
文章编号:0253-4193(2005)06-0154-08
收稿时间:2004-11-18
修稿时间:2004-11-182005-09-21

Gynogenetic diploid induced by suppression of first cleavage of Chlamys farreri
PAN Ying,LI Qi,YU Rui-hai and WANG Ru-cai.Gynogenetic diploid induced by suppression of first cleavage of Chlamys farreri[J].Acta Oceanologica Sinica (in Chinese),2005,27(6):154-161.
Authors:PAN Ying  LI Qi  YU Rui-hai and WANG Ru-cai
Institution:1.Fisheries College, Ocean University of China, Qingdao 266003, China;College of Animal Science and Technology, Guangxi University, Nanning 530005, China2.Fisheries College, Ocean University of China, Qingdao 266003, China
Abstract:Conditions required for the induction of gynogenetic diploid by suppression of first cleavage of Chlamys farreri were examined using treatments of cytochalasin B(0.5 g/cm~3;CB) and 6-dimethylaminopurine(60 g/cm~3;6-DMAP).Treatments with CB and 6-DMAP were effective in inhibiting not only meiosis but also mitosis,and the optimum time for the suppression of first cleavage began at 60~65 min with 20 and 15 min duration time after insemination.This resulted in 12.5% and 27.5% mitotic gynogenetic diploids(mitotic-G2N) respectively.Cytological observations indicated that 6-DMAP disrupted the spindle at mitosis and inhibited chromosome movement,resulting in the formation of one diploid female nucleus,while CB inhibited the cytokinesis of first cleavage but not the segregation of chromosomes.Although attempts to produce mitotic-G2N demonstrated that ploidy levels and the developmental rate of D-shaped larvae are low,the results of this study suggest the possibility of producing mitoticG2N Chalamys farreri.
Keywords:Chlamys farreri  gynogenetic diploid  6-DMAP  CB  cytology
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《海洋学报》浏览原始摘要信息
点击此处可从《海洋学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号