| 缢蛏（Sinonovacula constricta）GST和HSP90基因克隆及其在氨氮胁迫下的表达特征分析 |
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| 引用本文： | 张欢,董迎辉,姚韩韩,俞凯佳,胡月亚,林志华.缢蛏（Sinonovacula constricta）GST和HSP90基因克隆及其在氨氮胁迫下的表达特征分析[J].海洋学报,2020,42(4):66-78. |
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| 作者姓名： | 张欢 董迎辉 姚韩韩 俞凯佳 胡月亚 林志华 |
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| 作者单位： | 1.宁波大学 海洋学院，浙江 宁波 315823 |
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| 基金项目： | 国家现代贝类产业技术体系项目（CARS-49）；浙江省农业新品种选育重大科技专项（2016C02055-9）；国家水产种质资源平台运行服务项目（2019DKA30470）；宁波市“环境科学与工程”一流学科项目。 |
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| 摘 要： | 克隆获得缢蛏(Sinonovacula constricta)谷胱甘肽S-转移酶(Sc-GSTσ)和热休克蛋白90(Sc-HSP90)基因的cDNA全长，分析了它们的组织表达差异及其在氨氮胁迫下的表达特征。结果表明，Sc-GSTσ的全长cDNA为1 414 bp，含有639 bp的开放阅读框(Open Reading Frame，ORF)，编码212个氨基酸，Sc-GSTσ氨基酸序列与其他物种的GST氨基酸序列同源性为31.88%～43.40%；而Sc-HSP90的全长cDNA为2 752 bp，ORF为2 181 bp，编码726个氨基酸，其氨基酸序列与其他物种HSP90的氨基酸序列同源性为76.77%～87.05%。荧光定量PCR分析发现，Sc-GSTσ和Sc-HSP90在缢蛏各组织中均有表达，两者均在肝胰腺中表达量最高。氨氮胁迫后，Sc-GSTσ和Sc-HSP90 mRNA在肝胰腺中表达均显著上调(p<0.05)，表明氨氮胁迫引起机体的应激反应，2个基因可能参与机体解毒或防御过程。但胁迫后期表达量下降推测是机体的防御能力有限，不足以完全保护宿主免受应激诱导的细胞损伤。
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| 关 键 词： | 缢蛏 GST HSP90 氨氮 表达特征 |
| 收稿时间： | 2019/5/27 0:00:00 |
| 修稿时间： | 2019/8/21 0:00:00 |
Cloning of GST and HSP90 genes of Sinonovacula constricta and analysis of their expression characteristics under ammonia nitrogen stress |
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| Zhang Huan,Dong Yinghui,Yao Hanhan,Yu Kaiji,Hu Yuey,Lin Zhihua.Cloning of GST and HSP90 genes of Sinonovacula constricta and analysis of their expression characteristics under ammonia nitrogen stress[J].Acta Oceanologica Sinica (in Chinese),2020,42(4):66-78. |
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| Authors: | Zhang Huan Dong Yinghui Yao Hanhan Yu Kaiji Hu Yuey Lin Zhihua |
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| Institution: | 1.School of Marine Sciences, Ningbo University, Ningbo 315823, China2.Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China |
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| Abstract: | The full-length cDNA of glutathione S-transferase (Sc-GSTσ) and heat shock protein 90 (Sc-HSP90) genes were cloned from Sinonovacula constricta and their expression characteristics under ammonia nitrogen stress were analyzed. The results show that the full-length cDNA of Sc-GSTσ was 1 414 bp, and containing 639 bp open reading frame (ORF), encoding 212 amino acid polypeptides. The homology of amino acid sequence of Sc-GSTσ with other species’ GST amino acid sequence was 31.88%?43.40%. The full-length cDNA of Sc-HSP90 was 2 752 bp, ORF was 2 181 bp, encoding 726 amino acids. The amino acid sequence was 76.77%?87.05% homology with other species. Quantitative analysis showed that Sc-GSTσ and Sc-HSP90 genes were expressed in all tested tissues, the strongest expression being in the digestive gland. After exposure to ammonia, the mRNA expression of Sc-GSTσ and Sc-HSP90 were significantly up-regulated (p<0.05) in the digestive gland, indicating that ammonia stress induced stress response, both GST and HSP90 may be participate the process of detoxification or defense. However, the decrease of expression in the later period of stress is presumed to be due to the organism have limited ability to defense, which is not enough to protect the host from stress-induced cell damage. |
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| Keywords: | Sinonovacula constricta GST HSP90 ammonia nitrogen expression characteristics |
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