| 军曹鱼（Rachycentron canadum）幼鱼对环境低氧胁迫氧化应激与能量利用指标的响应 |
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| 引用本文： | 李洪娟,陈刚,郭志雄,王维政,黄建盛,曾泽乾.军曹鱼（Rachycentron canadum）幼鱼对环境低氧胁迫氧化应激与能量利用指标的响应[J].海洋学报,2020,42(4):12-19. |
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| 作者姓名： | 李洪娟 陈刚 郭志雄 王维政 黄建盛 曾泽乾 |
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| 作者单位： | 广东海洋大学 水产学院，广东 湛江 524088 |
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| 基金项目： | 现代农业产业技术体系专项资金资助（CARS-47）；广东海洋大学大学生创新创业训练计划项目（CXXL2018131）；南方海洋科学与工程广东省实验室（湛江）（ZJW-2019-06）；2018年广东海洋大学起航计划项目（QHJHZR201818）。 |
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| 摘 要： | 本文探究环境低氧对军曹鱼(Rachycentron canadum)氧化应激和能量利用指标的影响，为军曹鱼的健康养殖提供参考依据。通过设置低氧胁迫–恢复实验，将军曹鱼幼鱼(平均体质量(220.67±20.73)g)在低氧((2.64±0.25)mg/L)胁迫3 h及复氧((6.34±0.15)mg/L)8 h、24 h和48 h后，测定其肝脏和肌肉组织的氧化应激与能量利用指标。结果显示，低氧胁迫后，肝脏中丙二醛（Malondialdehyde，MDA)、过氧化氢酶(Catalase，CAT)和谷胱甘肽还原酶(Glutathione Reductase，GR)活力均显著低于对照组(p<0.05)，乳酸脱氢酶(Lactate Dehydrogenase，LDH)活性显著高于对照组(p <0.05)；肌肉中MDA和脂质过氧化物(Lipid Peroxidase，LPO)活性均显著低于对照组(p<0.05)，超氧化物歧化酶(Superoxide Dismutase，SOD)和LDH活性均显著高于对照组(p<0.05)；肌糖原和肝糖原含量极显著低于对照组(p<0.01)。复氧过程中，肝脏和肌肉中MDA、LPO、SOD、CAT、谷胱甘肽过氧化物酶(Glutathione Peroxidase，GPx)和GR含量均出现不同程度的升高；肝糖原在复氧24 h后显著高于对照组(p<0.05)，复氧48 h后显著低于对照组(p<0.05)；肌糖原在复氧8 h、24 h和48 h后均显著低于对照组(p<0.05)。研究表明，低氧胁迫能够对军曹鱼幼鱼机体造成一定的氧化损伤，肝脏和肌肉组织的酶活力和能量供应发生变化；低氧胁迫后的再复氧环境，对机体造成更为强烈的氧化损伤，可通过自身生理调节逐渐恢复到正常水平。
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| 关 键 词： | 军曹鱼 低氧胁迫 氧化应激 能量利用 |
| 收稿时间： | 2019/6/28 0:00:00 |
| 修稿时间： | 2019/10/17 0:00:00 |
Oxidative stress and energy utilization responses of juvenile cobia (Rachycentron canadum) to environmental hypoxia stress |
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| Li Hongjuan,Chen Gang,Guo Zhixiong,Wang Weizheng,Huang Jiansheng,Zeng Zeqian.Oxidative stress and energy utilization responses of juvenile cobia (Rachycentron canadum) to environmental hypoxia stress[J].Acta Oceanologica Sinica (in Chinese),2020,42(4):12-19. |
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| Authors: | Li Hongjuan Chen Gang Guo Zhixiong Wang Weizheng Huang Jiansheng Zeng Zeqian |
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| Institution: | College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China |
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| Abstract: | This study investigated the oxidative stress and energy utilization responses of juvenile cobia (Rachycentron canadum) to environmental hypoxia stress, and provided reference for the healthy cultivation of cobia. Through the hypoxia stress-reoxygenation test, the oxidative stress and energy utilization indexes of liver and muscle tissues were measured after hypoxia ((2.64±0.25)mg/L) stress for 3 h and reoxygenation ((6.34±0.15)mg/L) stress for 8 h, 24 h and 48 h. The results showed that after hypoxia stress, the activity of malondialdehyde (MDA), catalase (CAT) and glutathione reductase (GR) in the liver were significantly lower than that in the control group (p<0.05), and the activity of lactate dehydrogenase (LDH) was significantly higher than that in the control group (p<0.05). MDA and lipid peroxidase (LPO) activities in muscle were significantly lower than those in control group (p<0.05), and superoxide dismutase (SOD) and LDH activities were significantly higher than those in control group (p<0.05). The contents of muscle glycogen and liver glycogen were significantly lower than those of control group (p<0.01). During reoxygenation, the contents of MDA, LPO, SOD, CAT, glutathione peroxidase (GPx) and GR in liver and muscle all increased differentially. Liver glycogen content was significantly higher than that of the control group 24 h after reoxygenation (p<0.05), and significantly lower than that of the control group 48 h after reoxygenation (p<0.05). Muscle glycogen content was significantly lower than that of control group after reoxygenation for 8 h, 24 h and 48 h (p<0.05). In conclusion, hypoxia stress can cause some oxidative damage to the body of cobia, and the enzyme activity and energy supply of liver and muscle tissues change. The reoxygenation environment after hypoxia stress causes more severe oxidative damage to the body, which can be gradually restored to the normal level through physiological regulation. |
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| Keywords: | Rachycentron canadum hypoxia stress oxidative stress energy utilization |
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