| 4种石斑鱼的分子遗传多样性和亲缘关系的RAPD分析 |
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| 引用本文： | 尹绍武,黄海,廖经球,张本,陈国华.4种石斑鱼的分子遗传多样性和亲缘关系的RAPD分析[J].海洋学报,2006,28(6):119-126. |
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| 作者姓名： | 尹绍武 黄海 廖经球 张本 陈国华 |
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| 作者单位： | 热带生物资源教育部重点实验室, 海南海口 570228;海南大学海洋学院, 海南海口 570228 |
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| 基金项目： | 海南省教育厅高校科学研究资助项目(HjKj200513)，海南省自然科学基金资助项目(80411)，海南大学科研资助项目(Kyjj0439)，农业科技成果转化资金项目(02EFN214601172) |
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| 摘 要： | 采用25个引物,对海南养殖的点带石斑鱼、广东养殖的斜带石斑鱼、南海西沙野生鲑点石斑鱼和蜂巢石斑鱼的基因组DNA进行了RAPD分析,并用UPGMA法对4种石斑鱼进行聚类分析.研究结果表明:(1)野生鲑点石斑鱼和蜂巢石斑鱼群体内遗传多样性较高,多态位点比率分别为58.07%和55.65%,平均遗传杂合度分别为0.1793和0.1622,表明南海野生石斑鱼遗传变异水平较高,种质资源状况良好;而养殖的点带石斑鱼和斜带石斑鱼遗传多样性偏低,多态位点比率分别为49.70%和40.38%,平均遗传杂合度分别为0.1349和0.1135,表明石斑鱼养殖群体遗传多样性水平有所下降,应及时采取适当的保护措施.(2)4种石斑鱼种间遗传距离及聚类分析表明,点带石斑鱼与斜带石斑鱼遗传距离最近(D=0.2570);鲑点石斑鱼与蜂巢石斑鱼遗传距离次之(D=0.5146);斜带石斑鱼与蜂巢石斑鱼的亲缘关系最远(D=0.5810).(3)在8个引物中,有区分4种石斑鱼的特异性片段,可用于这些石斑鱼的鉴定.
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| 关 键 词： | 石斑鱼 分子遗传多样性 亲缘关系 RAPD分析 |
| 文章编号： | 0253-4193（2006）06-0119-08 |
| 收稿时间： | 01 4 2006 12:00AM |
| 修稿时间： | 2006-01-042006-06-08 |
RAPD analysis of molecular genetic diversity and genetic relationship of four grouper species |
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| YIN Shao-wu,HUANG Hai,LIAO Jing-qiu,ZHANG Ben and CHEN Guo-hua.RAPD analysis of molecular genetic diversity and genetic relationship of four grouper species[J].Acta Oceanologica Sinica (in Chinese),2006,28(6):119-126. |
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| Authors: | YIN Shao-wu HUANG Hai LIAO Jing-qiu ZHANG Ben and CHEN Guo-hua |
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| Institution: | Key Laboratory of Tropical Biology Resources, Ministry of Education, Haikou 570228, China;College of Ocean, Hainan University, Haikou 570228, China |
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| Abstract: | The RAPD(randomly amplified polymorphic DNA) technology was applied to studying the genetic diversity and genetic relationship of four grouper species,including two raised population(Epinephelus malabaricus from Hainan,China and E.coioides from Guangdong,China)and two wild populations(E.firio and E.merra captured from the South China Sea.25 random primers were used to be analyzed,and the genetic relationship of these grouper species was analyzed by UPGMA.The results indicated that: first,the percentages of polymorphic loci of the wild populations of E.frio and E.merra were 58.07% and 55.65%,and the mean heterozygositys of the wild populations of E. frio and E.merra were 0.179 3 and 0.162 2,respectively,the level of genetic diversity of them was high and the genetic status was good.However,that of the raised populations of E.malabaricus and E.coioides was low.The percentages of polymorphic loci were(49.70%) and 40.38%,and the mean heterozygositys were 0.134 9 and 0.113 5 respectively,which indicated the level of genetic diversity of the raised grouper populations was declined,and it should be taken some remedial measures.Second,the interspecies genetic distance and clustering analysis showed that: among four grouper species,the genetic distance between E.malabaricus and E.coioides was minimum(0.257 0),E.frio and E.merra took second palace(0.514 6),E.coioides and E.firio was maximum(0.581 0).Finally,among the fragments generated by 8 primers,some specific fragments which can be used to identify these groupers,were found. |
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| Keywords: | groupers molecular genetic diversity relationship RAPD analysis |
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