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三种海产帘蛤目贝类的核型研究
引用本文:孙振兴,郭胜超,邵雁群,秦燕,杨爱国.三种海产帘蛤目贝类的核型研究[J].海洋学报,2004,26(1):88-94.
作者姓名:孙振兴  郭胜超  邵雁群  秦燕  杨爱国
作者单位:1.烟台师范学院, 生物科学与技术系, 山东, 烟台, 264025
基金项目:国家科技攻关计划资助项目(2001BA505B0505),山东省自然科学基金资助项目(Y2002D12).
摘    要:以大竹蛏、紫石房蛤和中国蛤蜊的鳃组织为材料,经注射植物血球凝集素和秋水仙素处理,低渗作用后,采用滴片法和压片法制片,对3种贝的核型进行了研究.结果表明,3种贝类的二倍体染色体数均为38条,大竹蛏的核型为26m+6sm+2st+4t,NF=0;紫石房蛤的核型为32m+2sm+4st/t,NF=2;中国蛤蜊的核型为20m+16sm+2st/t,NF=4.在3种贝类的染色体中均未发现随体及性染色体.

关 键 词:大竹蛏    紫石房蛤    中国蛤蜊    染色体    核型
文章编号:0253-4193(2004)01-0088-07
收稿时间:2003/3/20 0:00:00
修稿时间:7/4/2003 12:00:00 AM

Karyotypes of three species of the marine Veneroida molluscs
SUN Zhen-xing,GUO Sheng-chao,SHAO Yan-qun,QIN Yan and YANG Ai-guo.Karyotypes of three species of the marine Veneroida molluscs[J].Acta Oceanologica Sinica (in Chinese),2004,26(1):88-94.
Authors:SUN Zhen-xing  GUO Sheng-chao  SHAO Yan-qun  QIN Yan and YANG Ai-guo
Institution:1.Department of Biological Science and Biotechnology, Yatai Normal University, Yantai 264025, China2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
Abstract:The karyotypes of three species of marine Veneroida molluscs, Solen grandis Dunker, Saxidomus purpuratus Sowerby and Mactra chinensis Philippi were studied by using the adult gill tissues. The chromosomes were prepared through injection of phytohemagglutinin (PHA) and colchicine, hypotonic treatment, chopping air-dry, and squashing technology. The results show that the diploid chromosome numbers of the three Veneroida species are the same as 2n=38. The karyotype of Solen grandis is 26m+6sm+2st+4t, NF=70, of Saxidomus purpuratus is 32m+2sm+4st/t, NF=72, of Mactra chinensis is 20m+16sm+2st/t, NF=74. Satellite and sex chromosome were not found among the chromosomes of three species.
Keywords:Solen grandis Dunker  Saxidomus purpuratus Sowerby  Mactra chinensis Philippi  chromosome  karyotype
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