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HeLa细胞突起中微丝束的纳米分辨荧光成像
引用本文:陈丹妮,刘磊,于斌,牛憨笨.HeLa细胞突起中微丝束的纳米分辨荧光成像[J].海洋学报,2010,32(10):6948-6954.
作者姓名:陈丹妮  刘磊  于斌  牛憨笨
作者单位:华中科技大学光电子科学与工程学院,武汉 430074; 深圳大学光电工程学院,光电子器件与系统(教育部/广东省)重点实验室,深圳 518060;深圳大学光电工程学院,光电子器件与系统(教育部/广东省)重点实验室,深圳 518060;深圳大学光电工程学院,光电子器件与系统(教育部/广东省)重点实验室,深圳 518060;深圳大学光电工程学院,光电子器件与系统(教育部/广东省)重点实验室,深圳 518060
基金项目:国家自然科学基金(批准号:60878053)、广东省高等学校科技创新团队项目(批准号:06CXTD009)和深圳市科技计划基础研究计划(批准号:JC200903130329A)资助的课题.
摘    要:在Matlab编程环境下模拟了单分子定位显微的纳米分辨成像,在传统实验参数条件下,对不同间隔分子带模型进行了模拟成像.模拟结果表明,单分子定位显微方法可以区分中心相隔20 nm的两个分子带.同时,也分析了不同像元大小对单分子定位精度的影响.此外,通过单分子定位显微方法在IX71倒置荧光显微镜上实现了纳米分辨,系统极限分辨率,即半高全宽为48 nm.在该系统上,获得了HeLa细胞突起中微丝束结构的纳米分辨图像.从重构获得的图像中可以看到微丝束的直径为75—200 nm.

关 键 词:纳米分辨  单分子定位  HeLa细胞  微丝束  细胞突起

Nano-resolution imaging of filopodia in HeLa cells
Chen Dan-Ni,Liu Lei,Yu Bin and Niu Han-Ben.Nano-resolution imaging of filopodia in HeLa cells[J].Acta Oceanologica Sinica (in Chinese),2010,32(10):6948-6954.
Authors:Chen Dan-Ni  Liu Lei  Yu Bin and Niu Han-Ben
Institution:Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China; College of Optoelectronic Science and Engineering, Huazhong Universit;College of Optoelectronic Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China;College of Optoelectronic Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China;College of Optoelectronic Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
Abstract:Nano-resolution imaging based on single molecule localization is simulated with Matlab. A sample consisted of several lines of molecules are simulated imaged. The results demonstrate that two lines of molecules separated by 20 nm can be resolved. Effect of pixel size on the localization accuracy is also discussed here. Nano-resolution imaging is realized on an IX71 inverted fluorescent microscope with the resolution (FWHM) of 48 nm. Nano-resolution images of filopodias are reconstructed, in which filopodias with diameter of 75 nm are resolved.
Keywords:nano-resolution  single molecule localization  HeLa cell  microtube  filopodia
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