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人工饲料饲养的对虾肠道菌群和水体细菌区系的研究
引用本文:杨莺莺,李卓佳,林亮,郭志勋.人工饲料饲养的对虾肠道菌群和水体细菌区系的研究[J].热带海洋学报,2006,25(3):53-56.
作者姓名:杨莺莺  李卓佳  林亮  郭志勋
作者单位:1. 中国水产科学研究院南海水产研究所,广东,广州,510300
2. 广州市第六中学,广东,广州,510300
基金项目:广东省科技攻关项目;国家科技攻关项目
摘    要:对人工饲料饲养的凡纳滨对虾Litopenaeus vannamei成虾肠道菌群组成及其水体细菌区系组成进行了研究.分离纯化后的革兰氏阴性菌鉴定到种的水平.对虾肠道菌群主要由弧菌Vibrio spp.、希瓦氏菌属Shewnella spp.、嗜氢菌属Hydrogenophaga spp.、伯克霍尔德氏菌Burkholderia spp.、气单胞菌Aeromonas spp.、食酸菌Acidovorax spp.和芽孢杆菌Bacillus spp.组成.水体细菌区系主要包括弧菌Vibrio spp.、希瓦氏菌属Shewnella spp.、艾肯菌Empedobacter spp.、无色杆菌Achromobacter spp.和芽孢杆菌Bacillus spp..肠道菌群和水体细菌区系的优势菌是副溶血弧菌Vibrio parahaemolyticus和芽孢杆菌Bacillus spp..利用Shannon-Wiener指数进行多样性分析,发现虾肠道多样性指数(H)和均匀度(J)高于水体细菌区系,而丰度(D)较低.

关 键 词:虾肠道菌群  水体细菌区系
文章编号:1009-5470(2006)03-0053-04
收稿时间:2005-07-27
修稿时间:2005-12-02

Analyses on intestinal flora of cultured shrimp and water microbial flora
YANG Ying-ying,LI Zhuo-jia,LIN Liang,GUO Zhi-xun.Analyses on intestinal flora of cultured shrimp and water microbial flora[J].Journal of Tropical Oceanography,2006,25(3):53-56.
Authors:YANG Ying-ying  LI Zhuo-jia  LIN Liang  GUO Zhi-xun
Institution:1. South China Sea Fisheries Institute, CAFS, Guangzhou 510300, China; 2. Guangzhou No. 6 Senior High School, Guangzhou 510300, China
Abstract:Intestinal flora and water microbial flora of adult shrimp,Litopenaeus vannamei,were investigated under experimental conditions.The isolated strains were purified and the Gram-negative bacteria were identified at species level by Biolog rapid identification system.The isolated bacteria from intestinal flora belonged to the genera of Vibrio spp.,Shewnella spp.,Hydrogenophaga spp.,Burkholderia spp.,Aeromonas spp.,Acidovorax spp.,and Bacillus spp.The isolated bacteria from water microbial flora belonged to the genera of Vibrio spp.,Shewnella spp.,Empedobacter spp.,Achromobacter spp.,and Bacillus spp.Vibrio parahaemolyticus and Bacillus spp.were the dominant species of intestinal flora and water microbial flora.Shannon-Wiener index was used to analyze the species diversity.It was found that the diversity index(H) and the abundance(D) of the shrimp intestinal flora were higher than those of the water microbial flora,whereas the evenness(J) of the shrimp intestinal flora was lower than that of the water microbial flora.
Keywords:shrimp intestinal flora  water microbial flora
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