| 斑节对虾3个野生群体遗传多样性的微卫星标记分析 |
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| 引用本文： | 赵志英,梁丽运,白丽蓉.斑节对虾3个野生群体遗传多样性的微卫星标记分析[J].热带海洋学报,2018,37(3):65-72. |
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| 作者姓名： | 赵志英 梁丽运 白丽蓉 |
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| 作者单位： | 1. 海南省海洋与渔业科学院 海南 海口 5711262. 海南省热带海水养殖技术重点实验室 海南 琼海 571429 |
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| 基金项目： | 海南省应用技术研发与示范推广专项(ZDXM2015025) |
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| 摘 要： | 为探究不同地理条件下斑节对虾天然群体的遗传多样性和遗传分化水平, 选用14对简单重复序列(simple sequence repeat, SSR)特异引物对广东湛江、海南三亚、海南琼海的斑节对虾天然群体共计90尾个体进行微卫星标记分析, 每对引物检测出的等位基因数为2~4不等, 平均2.7。3个群体(湛江、三亚、琼海)平均有效等位基因分别为1.9081、1.9715和2.0185, 平均多态信息含量分别为0.3864、0.3926和0.4078, 总的平均期望杂合度(0.4605 )明显高于观测杂合度(0.4046), 表明3个群体遗传多样性水平为中等, 且存在杂合度缺失的现象。种群分化指数和遗传距离分析表明, 在不同群体间已产生了遗传分化, 但是分化较小; 三亚群体和琼海群体之间的亲缘关系最近, 而与湛江群体的亲缘关系最远。分子方差分析表明, 群体间的遗传变异指数为8%, 群体内个体间的遗传变异指数为92%, 基因分化主要发生在群体内, 而不是群体之间。
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| 关 键 词： | 斑节对虾 微卫星标记 群体 遗传多样性 遗传分化 |
| 收稿时间： | 2017-07-30 |
| 修稿时间： | 2017-10-17 |
Analysis of genetic diversity among three wild populations of Penaeus monodon using microsatellite marker |
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| Zhiying ZHAO,Liyun LIANG,Lirong BAI.Analysis of genetic diversity among three wild populations of Penaeus monodon using microsatellite marker[J].Journal of Tropical Oceanography,2018,37(3):65-72. |
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| Authors: | Zhiying ZHAO Liyun LIANG Lirong BAI |
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| Institution: | 1. Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126 China2. Hainan Key Laboratory of Tropical Marine Culture Technology, Qionghai 571429, China |
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| Abstract: | Genetic diversity and genetic differentiation among 90 individuals of Penaeus monodon from three wild populations (Zhanjiang, Sanya, and Qionghai) were investigated by using 14 pairs of simple sequence repeat (SSR) specific primers. The number of alleles detected by each pair of primers ranged from 2 to 4, with the average of 2.7. The mean polymorphism information contents of the three populations were 0.3864, 0.3926, and 0.4078, respectively; and the mean effective numbers of alleles were 1.9081, 1.9715 and 2.0185, respectively. The average expected heterozygosity (0.4605) of the three populations was significantly higher than the observed heterozygosity (0.40469), indicating moderate polymorphism and the existence of heterozygosity deficiency among the three populations. The results of genetic distances and genetic similarity showed that the genetic differentiation among the three populations was low; genetic variation occurred mainly within populations, not among populations. The genetic relationship of Sanya and Qionghai populations was the closest, while that of Sanya and Zhanjiang populations was the farthest. Analysis of molecular variance (AMOVA) revealed that most of the genetic variation resided within populations (92%), and less, among populations (8%), suggesting rich genetic diversity level within populations. |
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| Keywords: | Penaeus monodon microsatellite marker population genetic diversity genetic differentiation |
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