| 马氏珠母贝PfAQP4免疫功能初探 |
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| 引用本文： | 潘肖兰,刘惠茹,许濛,许瀚之,张华,何毛贤.马氏珠母贝PfAQP4免疫功能初探[J].热带海洋学报,2021,40(2):83-89. |
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| 作者姓名： | 潘肖兰 刘惠茹 许濛 许瀚之 张华 何毛贤 |
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| 作者单位： | 1.中国科学院南海海洋研究所, 中国科学院热带海洋生物资源与生态重点实验室, 广东省应用海洋生物学重点实验室, 中国科学院南海生态环境工程创新研究院, 广东 广州 5103012.中国科学院大学, 北京 100049 |
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| 基金项目： | 国家贝类产业技术体系岗位科学家项目(CARS-49);中国科学院南海生态环境工程创新研究院自主部署项目(ISEE2018PY03);以农产品为单元的广东省现代农业产业技术体系创新团队建设项目(贝藻产业)(2019KJ146);广东省科技计划项目(2017B0303014052)。 |
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| 摘 要： | 文章对马氏珠母贝水通道蛋白基因PfAQP4进行免疫功能分析。利用实时荧光定量分析免疫刺激和RNA干扰后PfAQP4及免疫相关基因的表达模式, 旨在为马氏珠母贝自然免疫研究提供新的资料。结果表明, 注射脂多糖LPS后, 在外套膜组织中PfAQP4 mRNA表达量在24h时显著上调(p<0.05), 在消化腺中PfAQP4 mRNA表达量先在12h时显著下调(p<0.05), 后在24h、36h和48h时显著上调(p<0.05)。注射聚肌胞poly(I:C)后, 在外套膜组织中PfAQP4 mRNA表达量在36h时和48h时显著上调(p<0.05), 在消化腺中PfAQP4 mRNA表达量在48h时显著上调(p<0.05)。RNA干扰PfAQP4表达后, 免疫相关基因CuZn-SOD在外套膜中的表达量显著下降(p<0.05), Pearson相关性分析显示PfAQP4与CuZn-SOD的表达存在显著正相关(r=0.818, p<0.001)。综上所述, PfAQP4参与马氏珠母贝的免疫应答。
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| 关 键 词： | 马氏珠母贝 水通道蛋白4基因 免疫刺激 RNA干扰 基因表达 |
| 收稿时间： | 2020-05-22 |
| 修稿时间： | 2020-08-16 |
Preliminary study on immunity function of aquaporin AQP4 in the pearl oyster Pinctada fucata martensii |
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| PAN Xiaolan,LIU Huiru,XU Meng,XU Hanzhi,ZHANG Hua,HE Maoxian.Preliminary study on immunity function of aquaporin AQP4 in the pearl oyster Pinctada fucata martensii[J].Journal of Tropical Oceanography,2021,40(2):83-89. |
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| Authors: | PAN Xiaolan LIU Huiru XU Meng XU Hanzhi ZHANG Hua HE Maoxian |
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| Institution: | 1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Applied Marine Biology, Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou 510301, China2. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | To identify the immunity function of Aquaporin of Pinctada fucata martensii(PfAQP4),the mRNA expression patterns of PfAQP4 and immune-related genes were detected by qPCR after immunostimulation and RNA interference.The results showed that the mRNA expression of PfAQP4 in the mantle was significantly up-regulated at 24 h(p<0.05),while that in the digestive gland was significantly down-regulated at 12 h,and increased at 24 h,36 h and 48 h after LPS injection(p<0.05).After poly(I:C)injection,the expression of PfAQP4 was significantly up-regulated in the mantle at 36 h and 48 h(p<0.05),and up-regulated at 48 h in the digestive gland(p<0.05).When PfAQP4 was knocked down by RNA interference,the mRNA expression of CuZn-SOD in the mantle was significantly down-regulated(p<0.05);a significant positive correlation of expression level between CuZn-SOD and PfAQP4 was found(r=0.818,p<0.001).We showed that PfAQP4 plays an important role in the immune response of P.f.martensii,which provide new data for the innate immunity of P.f.martensii. |
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| Keywords: | Pinctada fucata martensii aquaporin 4 immunoregulation RNA interference gene expression |
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