| 石斑鱼虹彩病毒SGIV在宿主细胞内依赖微管运动的行为特征 |
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| 引用本文： | 王立群,王劭雯,王宏达,秦启伟.石斑鱼虹彩病毒SGIV在宿主细胞内依赖微管运动的行为特征[J].热带海洋学报,2020,39(1):66-73. |
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| 作者姓名： | 王立群 王劭雯 王宏达 秦启伟 |
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| 作者单位： | 1. 中国科学院热带海洋生物资源与生态重点实验室, 中国科学院南海海洋研究所, 广东 广州 5103012. 中国科学院大学, 北京 1000493. 华南农业大学海洋学院, 广东 广州 5106424. 中国科学院长春应用化学研究所电分析化学国家重点实验室, 吉林 长春 130022 |
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| 基金项目： | 国家自然科学基金项目(41606183);广东省自然科学基金项目(2016A030310111);国家重点研发计划资助(2017YFA0505300);国家自然科学基金项目(21727816);国家自然科学基金项目(21721003);国家自然科学基金项目(21525314) |
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| 摘 要： | 病毒是非常微小的简单生物,不能独立生存,必须借助宿主细胞完成自身的繁衍。病毒侵染进入细胞后,通常借助于微管通过黏稠的细胞质运动到特定的复制位点。然而,有关病毒依赖微管运动行为的精细动态研究还比较少。石斑鱼虹彩病毒(Singapore grouper iridovirus,SGIV)为虹彩病毒科蛙病毒属的一个新种,是海水养殖鱼类的重要病毒性病原,对海水养殖业造成重大经济损失。利用单粒子示踪技术实时追踪了SGIV病毒粒子沿微管运动的行为,观察到SGIV在细胞边缘至微管中心之间的双向运动,最高瞬时速度约0.2μm·s^–1,均表现为主动运输。病毒粒子运动至微管交叉位置会减速迂回,而后或受限于此,平均运动速率约0.008μm·s^–1,或通过交叉处继续快速运动,最高瞬时速度为0.2μm·s^–1。同时,SGIV感染会影响微管的形态结构,随着SGIV感染,微管逐渐围绕细胞核和病毒加工厂形成环状结构。研究结果初步揭示了SGIV病毒和细胞微管之间相互作用的复杂过程,丰富了我们对虹彩病毒胞内生命活动的认识,有助于深入地理解海水鱼类虹彩病毒感染致病机理。
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| 关 键 词： | 石斑鱼虹彩病毒 单粒子示踪技术 微管 病毒运输 |
| 收稿时间： | 2019-03-03 |
| 修稿时间： | 2019-04-22 |
Dynamic characteristics of microtubule-dependent transport of Singapore Grouper Iridovirus in host cells |
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| WANG Liqun,WANG Shaowen,WANG Hongda,QIN Qiwei.Dynamic characteristics of microtubule-dependent transport of Singapore Grouper Iridovirus in host cells[J].Journal of Tropical Oceanography,2020,39(1):66-73. |
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| Authors: | WANG Liqun WANG Shaowen WANG Hongda QIN Qiwei |
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| Institution: | 1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China2. University of Chinese Academy of Sciences, Beijing 100049, China3. College of Marine Sciences, South China Agricultural University, Guangzhou 5106424. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun 130022, China |
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| Abstract: | Viruses are tiny intracellular parasites and rely on the cellular machinery for replication. Usually, internalized viruses hijack the microtubule for effectively moving from cell membrane to specific compartments for replication. However, the elaborate process of microtubule-dependent transport of viruses in live cells remains unclear. Singapore grouper iridovirus (SGIV) belongs to genus Ranavirus, family Iridoviridae, which is an important viral pathogens marine fish, and causes serious economic losses. In this study, different microtubule-dependent behaviors of SGIV were analyzed by the single-particle tracking technique. We found that some SGIV particles showed bi-directional and active motions from cell periphery to the microtubule organizing center (MTOC), with the highest speed reaching 0.2μm·s-1. When reaching the microtubule intersection, SGIV particles slowed down. Then, some virus was confined near the intersection, with an average speed of 0.008 μm·s-1. Other virus moved forward through the intersection, with a high speed of 0.2μm·s-1 again. In addition, SGIV infection could affect the morphology. The microtubule gradually formed a circular structure around the nucleus and viral factory during SGIV infection. These preliminary results reveal the complex interaction between SGIV and microtubule, further our understanding of intracellular activity of iridoviruses, and contribute to our understanding of the pathogenesis of iridoviruses infection. |
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| Keywords: | Singapore grouper iridovirus (SGIV) single-particle tracking technology microtubule virus transport |
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