| 注射生物胺对凡纳滨对虾免疫指标的影响 |
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| 引用本文： | 胡发文,潘鲁青,杨慧赞.注射生物胺对凡纳滨对虾免疫指标的影响[J].热带海洋学报,2007,26(5):64-68. |
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| 作者姓名： | 胡发文 潘鲁青 杨慧赞 |
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| 作者单位： | 中国海洋大学,海水养殖教育部重点实验室,山东,青岛,266003 |
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| 基金项目： | 教育部跨世纪优秀人才培养计划;山东省科技兴海项目 |
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| 摘 要： | 研究了注射生物胺(多巴胺、5-羟色胺)对凡纳滨对虾Litopenaeus vanna mei免疫指标的影响。结果表明,注射生物胺(10-9mol.尾-1)对凡纳滨对虾血细胞数量、血淋巴酚氧化酶活力、溶菌和抗菌活力影响显著(p<0.05),而对照组各免疫指标无明显变化;在实验时间内,注射生物胺处理组各免疫指标呈明显峰值变化,血细胞数量均在3h达到最小值,血淋巴酚氧化酶活力和溶菌、抗菌活力均分别于6h时达到最大值和最小值,且多巴胺处理组各免疫指标比5-羟色胺处理组变化更明显,其中大、小颗粒细胞和血淋巴酚氧化酶活力在12h后保持稳定,总血细胞和透明细胞数量18h后趋于稳定,血淋巴溶菌和抗菌活力在24h后稳定,而且稳定后各免疫指标与对照组均无显著差异。
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| 关 键 词： | 多巴胺 5-羟色胺 凡纳滨对虾Litopenaeus vannamei 免疫指标 |
| 文章编号： | 1009-5470（2007）05-0064-05 |
| 收稿时间： | 2006-09-27 |
| 修稿时间： | 2007-01-22 |
Effects of injecting biogenic amines on immune parameters of white shrimp Litopenaeus vannamei |
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| HU Fa-wen,PAN Lu-qing,YANG Hui-zan.Effects of injecting biogenic amines on immune parameters of white shrimp Litopenaeus vannamei[J].Journal of Tropical Oceanography,2007,26(5):64-68. |
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| Authors: | HU Fa-wen PAN Lu-qing YANG Hui-zan |
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| Abstract: | Effects of injecting biogenic amines(dopamine,5-hydroxytryptamine) on the immune parameters of white shrimp Litopenaeus vannamei were studied.The results showed that injection of biogenic amines had significant effects on haemocyte count,phenoloxidase activity,bacteriolytic activity and antibacterial activity of haemolymph of white shrimp Litopenaeus vannamei(p<0.05),while no significant difference of the immune parameters was observed in the control group(0.85% NaCl).During the experiment,all immune parameters in the experimental groups changed significantly,haemocyte count decreased and became lowest at the 3rd hour,phenoloxidase activity peaked at the 6th hour,bacteriolytic activity and antibacterial activity of haemolymph became lowest at the 6th hour.Parameters in the experimental group with dopamine injection changed more markedly than those in the one with 5-Hydroxytryptamine injection.The counts of granular and semi-granular cells and the phenoloxidase activity became stable after 12h,the counts of hyaline cells and total haemocyte became stable after 18h,and the bacteriolytic activity and antibacterial activity became stabled after 24h.All parameters of the experiment groups had no marked differentia from those of the control group after they were stable. |
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| Keywords: | dopamine 5-Hydroxytryptamine Litopenaeus vannamei immuneparameter |
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