| 三份南海岛礁珊瑚砂样品中可培养细菌多样性 |
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| 引用本文： | 李存,崔林青,杨红强,龙丽娟,田新朋.三份南海岛礁珊瑚砂样品中可培养细菌多样性[J].热带海洋学报,2022,41(2):149-158. |
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| 作者姓名： | 李存 崔林青 杨红强 龙丽娟 田新朋 |
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| 作者单位： | 1. 中国科学院热带海洋生物资源与生态重点实验室(中国科学院南海海洋研究所), 广东 广州 5103012. 中国科学院边缘海与大洋地质重点实验室(中国科学院南海海洋研究所), 广东 广州 5103013. 南方海洋科学与工程广东省实验室(广州), 广东 广州 5114584. 中国科学院大学, 北京 100049 |
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| 基金项目： | 中国科学院战略性先导科技专项(XDA19060301);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0402);广东省“珠江人才计划”本土创新科研团队项目(2019BT02Y262) |
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| 摘 要： | 岛礁珊瑚砂环境中存在着大量未被培养和利用的微生物资源, 微生物多样性研究是了解微生物生态功能、开发和利用微生物资源的基础。文章采用多种寡营养培养基选择性分离南海岛礁珊瑚砂中的可培养细菌, 共获得纯培养细菌菌株349株, 通过16S rRNA基因序列分析, 发现它们隶属于4门(Actinobacteria、Proteobacteria、Firmicutes和Bacteroidetes)、6纲、26目、43科、73属、134种, 可培养细菌的优势类群为放线菌门, 占所有分离菌株数量的60%; 而且还发现18个16S rRNA 基因序列相似性低于97%的潜在新种。本研究使用改良优化的寡营养培养基进行分离, 较好地显示出样品中微生物的群落组成, 且获得了大量潜在的稀有新物种资源。研究结果表明, 岛礁珊瑚砂样品的可培养细菌资源十分丰富、细菌群落所涉及的生态功能完整、潜在新种比例较高, 为后期岛礁微生物资源挖掘打下了良好的基础, 也为后期开发应用积累了丰富且稀有的菌种资源。
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| 关 键 词： | 南海岛礁 寡营养培养基 可培养细菌多样性 微生物资源 |
| 收稿时间： | 2021-04-15 |
| 修稿时间： | 2021-05-12 |
Diversity of cultured bacteria isolated from three coral reef sediments in South China Sea |
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| LI Cun,CUI Linqing,YANG Hongqiang,LONG Lijuan,TIAN Xinpeng.Diversity of cultured bacteria isolated from three coral reef sediments in South China Sea[J].Journal of Tropical Oceanography,2022,41(2):149-158. |
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| Authors: | LI Cun CUI Linqing YANG Hongqiang LONG Lijuan TIAN Xinpeng |
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| Abstract: | There are a large number of uncultured microbial resources in coral reef environments. Microbial diversity research will help us understand their ecological functions, and also help develop and utilize microbial resources. In this study, a total of 349 pure bacterial strains were obtained by selective isolation media with multiple oligotrophic components, from three samples collected from three coral reef islands in the South China Sea. Based on 16S rRNA gene sequence analysis, we found the cultivable bacteria belong to four phyla of Actinobacteria, Proteobacteria, Firmicutes and Bacteroidetes; and they spread into six class, 26 orders, 43 families, 73 genera, and 134 species. There are 18 potential new taxa with 16S rRNA gene sequence similarities less than 97%. The dominant group is Actinobacteria, which accounts for 60% of all isolates. In this study, the improved and optimized oligotrophic media were used, which showed better isolation results including the composition of the microbial community and rare or new taxa in every sample. The results showed that the cultivable microbial resources are rich and diverse in coral reef island environments of the South China Sea, and the microbial community has comprehensive functions for material and energy metabolism; and the proportion is higher to obtain potential new taxa. This research has laid a good foundation for the methods to mine microbial resources in coral reef islands; it also accumulated abundant and rare microbial resources for the deep development and application in the future. |
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| Keywords: | Coral reef island oligotrophic medium cultivable bacterial diversity microbial resources |
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