| 牡蛎酶解物减轻5-FU对荷瘤小鼠肠黏膜损伤的作用研究* |
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| 引用本文： | 叶子晴,赵祥弹,蔡冰娜,万鹏,陈华,潘剑宇.牡蛎酶解物减轻5-FU对荷瘤小鼠肠黏膜损伤的作用研究*[J].热带海洋学报,2022,41(5):105-116. |
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| 作者姓名： | 叶子晴 赵祥弹 蔡冰娜 万鹏 陈华 潘剑宇 |
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| 作者单位： | 1.中国科学院南海海洋研究所, 中国科学院热带海洋生物资源与生态重点实验室, 广东 广州 5103012.中国科学院大学, 北京 1000493.南方海洋科学与工程广东省实验室(广州), 广东 广州 5114584.中国科学院南海生态环境工程创新研究院, 广东 广州 510301 |
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| 基金项目： | 国家重点研发计划(2018YFC0311202);广东省海洋经济发展专项(GDNRC[2020]038);广东省海洋经济发展专项(GDNRC[2020]036);广东省自然科学基金(2018A030313088);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0406);广东省重点领域研发计划(2020B1111030004);中国科学院南海生态环境工程创新研究院自主部署项目(ISEE2021PY05) |
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| 摘 要： | 文章以牡蛎肉为原料, 采用胰蛋白酶水解法制备获得牡蛎胰蛋白酶水解物(trypsin hydrolysates of oyster, OYH), 分析了其氨基酸组成和相对分子量分布, 并初步探究了OYH在减轻化疗药物5-氟尿嘧啶(5-fluorouracil, 5-FU)对S180荷瘤小鼠肠黏膜损伤方面的作用效果。结果表明: OYH中的必需氨基酸占总氨基酸的36.44%, 且谷氨酸含量最高, 其分子质量主要集中在1kDa以下, 所占比例为92.18%。5-FU单独使用组的肿瘤抑制率为31.00%; 与模型组相比, OYH-L+5-FU组、OYH-M+5-FU组和OYH-H+5-FU组小鼠肿瘤抑制率明显提高(p<0.001), 分别为38.24%、46.27%和47.57%, 其中OYH-M+5-FU组和OYH-H+5-FU组较5-FU单独使用有明显提高(p<0.01, p<0.001)。同时, OYH还能有效减轻5-FU给药期间造成的肠道黏膜损伤, 与5-FU组相比, OYH-L+5-FU组、OYH-M+5-FU组和OYH-H+5-FU组小鼠的小肠绒毛高度、隐窝深度、黏膜厚度和绒毛表面积均显著增加(p<0.01, p<0.001), 明显改善小肠隐窝和上皮绒毛的状态, 维持肠黏膜屏障的完整。此外, OYH-L+5-FU组、OYH-M+5-FU组和OYH-H+5-FU组的S180荷瘤小鼠与模型组相比, 中性粒细胞含量降低, 淋巴细胞含量增加(p<0.05, p<0.01), OYH-H+5-FU组能显著增加机体总蛋白、白蛋白以及免疫球蛋白含量(p<0.05, p<0.01, p<0.001), 改善机体免疫功能、营养状况以及调节血清免疫抗体水平。结果显示牡蛎肉的胰蛋白酶水解物具有作为化疗过程辅助治疗营养食品, 起到肠黏膜保护和提升机体耐受力的应用潜力。
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| 关 键 词： | 牡蛎酶解物 化疗 肿瘤抑制 肠黏膜保护 免疫调节 |
| 收稿时间： | 2021-11-10 |
| 修稿时间： | 2021-12-21 |
Oyster hydrolysates alleviate 5-fluorouracil-induced intestinal mucosal injury on S180 tumor-bearing mice* |
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| YE Ziqing,ZHAO Xiangdan,CAI Bingna,WAN Peng,CHEN Hua,PAN Jianyu.Oyster hydrolysates alleviate 5-fluorouracil-induced intestinal mucosal injury on S180 tumor-bearing mice*[J].Journal of Tropical Oceanography,2022,41(5):105-116. |
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| Authors: | YE Ziqing ZHAO Xiangdan CAI Bingna WAN Peng CHEN Hua PAN Jianyu |
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| Institution: | 1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China2. University of Chinese Academy of Sciences, Beijing 100049, China3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China4. Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou 510301, China |
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| Abstract: | In this study, oyster meat was used as the raw material to produce trypsin hydrolysates of oyster (OYH). The amino acid composition and relative molecular weight distribution of OYH were then analyzed. The effect of OYH on reducing 5-fluorouracil (5-FU) induced intestinal mucosal injury on S180 tumor-bearing mice was preliminarily investigated. The results showed that the essential amino acids in the total amino acids of OYH accounted for 36.44 %, and content of glutamic acid was the highest. The molecular weight of OYH was mostly below 1 kDa, accounting for 92.18 %. The results showed that the tumor inhibition ratio of 5-FU group was 31.00 %. Compared with model group, the tumor inhibition ratio of mice in OYH-L+5-FU, OYH-M+5-FU and OYH-H+5-FU group were significantly increased (p < 0.001) with the percentage of 38.24 %, 46.27 % and 47.57 %, respectively. Tumor inhibition ratios of OYH-M+5-FU and OYH-H+5-FU group were greatly higher than 5-FU (p < 0.01, p < 0.001). Meanwhile, OYH can alleviate intestinal mucosal injury caused by 5-FU chemotherapy. Compared with 5-FU alone treatment, OYH-L+5-FU, OYH-M+5-FU and OYH-H+5-FU group significantly increased the villus height, crypt depth, mucosa thickness and villus surface area of small intestine (p < 0.01, p < 0.001), which indicated that OYH can improve the state of intestinal crypt and epithelial villi, maintain the integrity of the intestinal mucosal barrier during chemotherapy. In addition, compared with model group, the percentage of neutrophils was decreased but the percentage of lymphocytes was increased in OYH-L+5-FU, OYH-M+5-FU and OYH-H+5-FU group (p < 0.05, p < 0.01). And the OYH-H+5-FU group can largely increase the levels of total protein, albumin and immune globulin in serum (p < 0.05, p < 0.01, p < 0.001), which showed that OYH play a role in the immunologic function, nutritional improvement and level of immune antibody regulation in serum immunoregulation. In conclusion, trypsin hydrolysates of oyster meat have the potential for becoming an adjuvant nutritional food in the process of chemotherapy, protecting intestinal mucosa and improving body tolerance. |
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| Keywords: | oyster hydrolysates chemotherapy anti-tumor intestinal mucosal protection immune regulation |
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