| 基于形态学和DNA分子鉴定的珠江口浮游动物群落结构比较研究 |
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| 引用本文： | 尹天齐,王庆,杨宇峰,岑竞仪.基于形态学和DNA分子鉴定的珠江口浮游动物群落结构比较研究[J].热带海洋学报,2022,41(3):172-185. |
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| 作者姓名： | 尹天齐 王庆 杨宇峰 岑竞仪 |
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| 作者单位： | 1.暨南大学生命科学技术学院, 广东 广州 5106322.南方海洋科学与工程广东省实验室, 广东 珠海 519000 |
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| 基金项目： | 广东省自然科学基金面上项目(2022A1515011387);广东省自然科学基金面上项目(2021A1515010814) |
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| 摘 要： | 于2019年12月使用4种不同型号网具采集了珠江口浮游动物, 进行形态学鉴定和DNA分子鉴定, 分析珠江口浮游动物的群落结构特征, 并比较不同调查方法对浮游动物丰度和生物量结果的影响。形态学镜检鉴定浮游动物36种(类), 其中浮游幼虫6类。浅水I型浮游生物网采集的浮游动物平均丰度为115±96ind.·m-3, 平均生物量为0.21±0.14g·m-3; 浅水Ⅱ型浮游生物网采集的浮游动物平均丰度为3536±2444ind.·m-3, 平均生物量为0.56±0.33g·m-3; 浅水Ⅲ型浮游生物网采集的浮游动物平均丰度为4314±4172ind.·m-3, 平均生物量为0.50±0.25g·m-3; 25#浮游生物网采集的浮游动物平均丰度为6741±3826ind.·m-3, 平均生物量为4.33±3.42g·m-3。研究结果表明网具孔径大小对浮游动物研究结果具有重要影响, 三个站点水体DNA样品注释出15种浮游动物; 使用浅水Ⅱ型网采集的DNA样品注释出19种浮游动物; 镜检样品鉴定浮游动物17种。水体DNA样品能检测出更多的微型浮游动物如原生动物等; 网采样品能过滤更多的水样, 有利于采集更多的大中型浮游动物, 更能充分反映优势类群如桡足类的种类和数量。研究结果表明, 水体DNA可检出浮游幼虫和原生动物等较难镜检鉴别的种类, 采用不同型号网具采集浮游动物可以更全面地反映研究海域浮游动物的群落结构特征。多种调查方法的结合有助于全面了解研究海域的生态环境状况。
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| 关 键 词： | 珠江口 浮游动物 粒径 DNA鉴定 |
| 收稿时间： | 2021-11-05 |
| 修稿时间： | 2021-12-15 |
Comparative study on zooplankton community structure in Pearl River Estuary based on morphological and DNA identification |
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| YIN Tianqi,WANG Qing,YANG Yufeng,CEN Jingyi.Comparative study on zooplankton community structure in Pearl River Estuary based on morphological and DNA identification[J].Journal of Tropical Oceanography,2022,41(3):172-185. |
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| Authors: | YIN Tianqi WANG Qing YANG Yufeng CEN Jingyi |
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| Institution: | 1. College of Life Science and Technology, Jinan University, Guangzhou 510632, China2. Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China |
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| Abstract: | Zooplankton samples in the Pearl River Estuary were collected in December 2019 using four different types of plankton nets. We analyzed the characteristics of zooplankton community structure and compared the effects of different investigation methods on the study of zooplankton community structure based on morphological identification and DNA monitoring. Thirty-six species of zooplankton including six species of planktonic larvae were identified by morphological identification. The average abundance of zooplankton collected by the I type of plankton net for the shallow water was 115±96 ind·m-3, and the average biomass was 0.21±0.14g·m-3. The average abundance of zooplankton collected by the Ⅱ type of plankton net for the shallow water was 3536±2444ind·m-3, and the average biomass was 0.56±0.33 g·m-3. The average abundance of zooplankton collected by the Ⅲ type of plankton net for the shallow water was 4314±4172 ind.·m-3, and the average biomass was 0.50±0.25 g·m-3. The average abundance of zooplankton collected by 25# plankton net was 6741±3826 ind·m-3, and the average biomass was 4.33±3.42 mg·m-3. Results show that the mesh size of plankton net has an important impact on the results of zooplankton. Water DNA samples were collected from three sites annotated 15 species of zooplankton; DNA samples were collected by the Ⅱ type of plankton net from three sites annotated 19 species of zooplankton; and 17 species of zooplankton were identified by morphology at the three sites. Results show that water DNA samples can detect micro-zooplankton, such as protozoa. DNA samples collected by the plankton net can filter much water, which is conducive to collecting more macro- and meso-sized zooplankton, and can fully reflect the species and abundance of dominant groups such as copepods. Water DNA samples can be used to detect species that are difficult to be identified, such as planktonic larvae and protozoa. Zooplankton collected with different types of plankton nets can comprehensively reflect the characteristics of zooplankton community structure in the study area. The combination of various investigation methods is helpful to comprehensively understand the ecological environment in the study area. |
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| Keywords: | Pearl River Estuary zooplankton particle size DNA identification |
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