| 广东省粤西地区凡纳滨对虾3种主要病毒的PCR与LAMP检测及流行病学特点分析 |
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| 引用本文： | 江晓,任春华,胡超群,赵哲.广东省粤西地区凡纳滨对虾3种主要病毒的PCR与LAMP检测及流行病学特点分析[J].热带海洋学报,2011(6). |
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| 作者姓名： | 江晓 任春华 胡超群 赵哲 |
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| 作者单位： | 中国科学院南海海洋研究所;中国科学院研究生院; |
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| 基金项目： | 国家农业公益性行业(农业)科研专项(200803012); 国家虾现代产业技术体系(nycytx-46); 广东省海洋渔业成果推广项目(A200901B03); 广东省-中国科学院合作项目(2009B091300088) |
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| 摘 要： | 应用聚合酶链式反应(Polymerase Chain Reaction,PCR)和环介导等温扩增(Loop-Mediated Isothermal Ampli-cation,LAMP)两种检测技术分别对2009年广东省粤西地区养殖体系中凡纳滨对虾Litopenaeus vannamei白斑综合症病毒(White Spot Syndrome,WSSV)、桃拉病毒(Taura Syndrome Virus,TSV)和传染性皮下及造血组织坏死病毒(Infectious Hypodermal and Hematopoietic Necrosis Virus,IHHNV)的携带情况进行了调查。结果显示,WSSV和TSV的携带率较高,是该地区凡纳滨对虾的主要流行病毒种类;LAMP检测方法与PCR方法具有相当的灵敏度和特异性,但LAMP检测方法更加简单方便、快速且成本低。
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| 关 键 词： | 凡纳滨对虾 白斑综合症病毒 传染性皮下及造血组织坏死病毒 桃拉病毒 聚合酶链式反应 环介导等温扩增 检测 |
Epidemiological investigation of three kinds of major virus in Litopenaeus vannamei from western Guangdong Province |
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| JIANG Xiao,REN Chun-hua,HU Chao-qun,ZHAO Zhe .South China Sea Institute of Oceanology,CAS,Guangzhou ,China,.Graduate University of CAS,Beijing.Epidemiological investigation of three kinds of major virus in Litopenaeus vannamei from western Guangdong Province[J].Journal of Tropical Oceanography,2011(6). |
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| Authors: | JIANG Xiao REN Chun-hua HU Chao-qun ZHAO Zhe South China Sea Institute of Oceanology CAS Guangzhou China Graduate University of CAS Beijing |
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| Institution: | JIANG Xiao1,2,REN Chun-hua1,HU Chao-qun1,ZHAO Zhe1 1.South China Sea Institute of Oceanology,CAS,Guangzhou 510301,China,2.Graduate University of CAS,Beijing 100049 |
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| Abstract: | In order to investigate the infected rates of White Spot Syndrome Virus(WSSV),Taura Syndrome Virus(TSV) and Infectious Hypodermal and Hematopoietic Necrosis Virus(IHHNV) among aquatic Litopenaeus vannamei,we tested the samples from western Guangdong Province using Polymerase Chain Reaction(PCR) and Loop-mediated Isothermal Amplication(LAMP).The results show that there were higher infected rates of WSSV and TSV,which suggests these two viruses were the dominant pathogens in this region.These data reveal that... |
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| Keywords: | Litopenaeus vannamei White Spot Syndrome Virus Infectious Hypodermal and Hematopoietic Necrosis Virus Taura Syndrome Virus detection |
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