| 海萝种藻冷冻保存对其释放孢子量及孢子附着的影响 |
| |
| 引用本文： | 陈素文,吴进锋,陈利雄.海萝种藻冷冻保存对其释放孢子量及孢子附着的影响[J].热带海洋学报,2012,31(1):67-71. |
| |
| 作者姓名： | 陈素文 吴进锋 陈利雄 |
| |
| 作者单位： | 中国水产科学研究院南海水产研究所 |
| |
| 基金项目： | 广东省科技计划项目(2006B20201061);广东省海洋渔业科技推广专项(A200899E01);中国水产科学研究院南海水产研究所中央级公益性科研院所专项资金项目(2007TS01,2010TS02) |
| |
| 摘 要： | 为了研究海萝的非繁殖季节孢子育苗方法,进行了2个实验来研究海萝种藻冷冻保存对其释放孢子量及孢子附着的影响。1)6组不同风干程度海萝种藻在18℃冷冻保存6个月后取出在16℃水中进行孢子的释放,种藻风干到干重为原鲜重约25%的组,孢子的保存效果最好,该组种藻释放孢子量约为对照组(未经风干和保存的种藻)的77.6%,且孢子的附着率与对照组的差异性不显著;2)风干到重量为原鲜重约1/4的种藻分别保存于18℃、36℃、80℃,在保存8个月后,每一保存温度下的种藻分别在8℃、12℃、16℃、20℃水中释放孢子,种藻在水温12℃下释放的孢子量最多;保存于36℃、80℃的种藻较保存于18℃的释放孢子量多。本实验结果为海萝的非繁殖季节孢子育苗找到一种简便的方法,也为该属藻类的种质保存提供了参考。
|
| 关 键 词： | 海萝种藻 冷冻保存 孢子 |
| 收稿时间： | 2010-11-02 |
Effects of freezing storage of fertile Gloiopeltis furcata on its released spores’ number and spores’ attachment |
| |
| CHEN Su-wen,WU Jin-feng,CHEN Li-xiong.Effects of freezing storage of fertile Gloiopeltis furcata on its released spores’ number and spores’ attachment[J].Journal of Tropical Oceanography,2012,31(1):67-71. |
| |
| Authors: | CHEN Su-wen WU Jin-feng CHEN Li-xiong |
| |
| Institution: | South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China |
| |
| Abstract: | In order to study spore culture of Gloiopeltis furcata in non-reproduction season,two experiments were conducted on freezing storage of fertile Gloiopeltis furcata in terms of its released spores’ number and spores’ attachment.In the first experiment,six group of fertile Gloiopeltis furcata of different dry-degree was preserved at 18℃ for six months and then released at water temperature of 16℃.The results showed that the best preservation effect of fertile Gloiopeltis furcata was the group that dry weight was about 25% of its fresh weight.The released spores’ number of this group was about 77.6% of the control group(the fertile Gloiopeltis furcata which was not dealed with air drying and preservation) and there was no significant difference in spores’ attachment between this group and the control group.In the second experiment,fertile Gloiopeltis furcata that dry weight was about 1/4 of its fresh weight was preserved at 18℃,36℃,and 80℃ for eight months and then released spores at water temperature of 8℃,12℃,16℃,and 20℃.The results showed maximum spores appeared at water temperature of 12℃ and fertile Gloiopeltis furcata that preserved at 36℃ and 80℃ released more spores than those preserved at 18℃.The two experiments showed a simple and convenient method for spore culture of Gloiopeltis furcata in non-reproduction season and would assist the preservation of germ plasm of Gloiopeltis. |
| |
| Keywords: | fertile Gloiopeltis furcata freezing storage spore |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《热带海洋学报》浏览原始摘要信息 |
| 点击此处可从《热带海洋学报》下载免费的PDF全文 |
|
