| 大亚湾紫红笛鲷野生群体遗传多样性的RAPD分析 |
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| 引用本文： | 牛文涛,蔡泽平.大亚湾紫红笛鲷野生群体遗传多样性的RAPD分析[J].海洋通报,2006,25(2):87-92. |
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| 作者姓名： | 牛文涛 蔡泽平 |
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| 作者单位： | 1. 中国科学院南海海洋研究所,广东,广州,510301;广东省实验动物监测所,广东,广州,510260
2. 中国科学院南海海洋研究所,广东,广州,510301 |
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| 基金项目： | 中国科学院知识创新工程重点方向项目(ZKCX2-211)，广东省科技攻关项目(2004B20301001) |
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| 摘 要： | 紫红笛鲷野生群体样品取自广东大亚湾海区,取背部肌肉,提取DNA,用RAPD技术检测遗传多样性。40个随机引物当中,有11个引物得到了条带清晰、位点较多且重复性好的电泳图谱。11个引物共得到76个条带,其中48个多态位点,多态位点比例为63.16%。平均遗传距离为0.2144。遗传多样性指数为0.1876。结果显示,大亚湾海区的紫红笛鲷具有较丰富的遗传多样性,工程建设以及人为捕捞等对紫红笛鲷的遗传结构影响不明显。在该海区进行紫红笛鲷的养殖,可以选择野生紫红笛鲷作为亲本培育种苗,但应该注意合理的保护,防止遗传多样性的丧失。
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| 关 键 词： | 大亚湾 紫红笛鲷 遗传多样性 随机扩增多态性DNA |
| 文章编号： | 1001-6392（2006）02-0087-06 |
| 收稿时间： | 2005-03-16 |
| 修稿时间： | 2005-03-162005-07-11 |
Genetic Diversity in the Wild Population of Lutjanus argentimaculatus in the Daya Bay by RAPD |
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| NIU Wentao,CAI Zeping.Genetic Diversity in the Wild Population of Lutjanus argentimaculatus in the Daya Bay by RAPD[J].Marine Science Bulletin,2006,25(2):87-92. |
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| Authors: | NIU Wentao CAI Zeping |
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| Institution: | 1 .South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, Guangdong, China; 2. Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510260, Guangdong, China |
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| Abstract: | Random amplified polymorphic DNA ( RAPD) technique was applied to assess the DNA polymorphism in the wild population of L utjanus argentimaculatus ( 8 individuals ) in the Daya Bay. Amplifications with 11 random primers selected from 40 primers gave 76 reproducible and stable bands. There were 48 polymorphic bands. The percentage of polymorphic loci (P) is about 63.16%. The genetic distance (D ) between the individuals is 0.214 4 on the average. The genetic diversity index (H ) is 0.187 6. The results show that the genetic diversity of L utjanus argentimaculatus in the Daya Bay is rich comparatively. The project constructions and the fishing activities have not caused obvious reduction in genetic diversity of L utjanus argentimaculatus.The wild Lutjanus argentimaculatus c an be used as a parent resource for aquiculture, but some safeguards should be taken to avoid the loss of genetic diversity. |
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| Keywords: | Daya Bay Lutjanus argentimaculatus genetic diversity RAPD ( randaomly amplified polymorphic DNA) |
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