| 渤海裸甲藻和链状亚历山大藻的麻痹性贝毒毒素分析 |
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| 引用本文： | 贺华,王学魁,孙之南,逄勃越,曹春晖,王广策,于仁诚.渤海裸甲藻和链状亚历山大藻的麻痹性贝毒毒素分析[J].海洋通报,2007,26(5):67-73. |
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| 作者姓名： | 贺华 王学魁 孙之南 逄勃越 曹春晖 王广策 于仁诚 |
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| 作者单位： | 1. 天津科技大学海洋科学与工程学院,天津,300457
2. 中国科学院海洋研究所,山东,青岛,266071 |
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| 摘 要： | 目的:分析渤海裸甲藻(Gymnodinium sp.)和链状亚历山大藻(Alexandrium catenella)的麻痹性贝毒毒素,为渤海天津海域的赤潮研究积累基础数据。方法:通过实验室培养裸甲藻和链状亚历山大藻,选取对数生长期、平台生长期的裸甲藻以及平台生长期的链状亚历山大藻,利用高效液相色谱法(HPLC)对这两种微藻进行麻痹性贝毒(PSP)毒素分析。结果:裸甲藻细胞内不含有麻痹性贝毒(PSP);链状亚历山大藻细胞内含有C毒素和GTX1-4毒素,该微藻每个细胞毒素含量约为10.81 fmol/cell。结论:裸甲藻细胞内虽不含有麻痹性贝毒(PSP),但不能排除其含有其它毒素的可能。链状亚历山大藻细胞内含有麻痹性贝毒(PSP),属于有毒微藻,需要对其进行密切监测。
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| 关 键 词： | 裸甲藻 链状亚历山大藻 麻痹性贝毒(PSP) 高效液相色谱(HPLC) |
| 文章编号： | 1001-6932（2007）05-0067-0007 |
| 修稿时间： | 2006-03-07 |
Toxin Analysis of Paralytic Shellfish Poisoning from Gymnodinium sp. and Alexandrium Catenella in Bohai Sea |
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| HE Hua,WANG Xuekui,SUN Zhinan,PANG Boyue,CAO Chunhui,WANG Guangce,YU Rencheng.Toxin Analysis of Paralytic Shellfish Poisoning from Gymnodinium sp. and Alexandrium Catenella in Bohai Sea[J].Marine Science Bulletin,2007,26(5):67-73. |
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| Authors: | HE Hua WANG Xuekui SUN Zhinan PANG Boyue CAO Chunhui WANG Guangce YU Rencheng |
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| Abstract: | This paper is aimed at accumulating the basic data on the red-tide through analyzing the paralytic shellfish poisoning (PSP) from Gymnodinium sp. and Alexandrium catenella in the Bohai Sea area of Tianjin. By culturing Gymnodinium sp. and Alexandrium catenella in the laboratory, we selected Gymnodinium sp. in the logarithm phase and flat stage and also Alexandrium catenella in the flat stage as objectives of our research. The toxins of paralytic shellfish poisoning were analyzed in the Gymnodinium sp. and Alexandrium catenella by using high performance liquid chromatograghy (HPLC). The results showed that Gymnodinium sp. does not contain paralytic shellfish poisoning (PSP) in cell; Alexandrium catenella contains C toxin and GTX1-4 toxin with a total toxin content of approximately 10.81 fmol/cell. It is concluded that Gymnodinium sp. does not contain paralytic shellfish poisoning (PSP) in cell, but we can not exclude the possibility of containing other toxins. Because of containing paralytic shellfish poisoning (PSP), Alexandrium catenella belongs to the poisonous microalgae and therefore we should make a close monitoring to it. |
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| Keywords: | Gymnodinium sp Alexandrium catenella paralytic shellfish poisoning (PSP) high performance liquid chromatograghy (HPLC) |
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