| 黄鳍鲷不同组织同工酶的研究 |
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| 引用本文： | 邓思平,刘楚吾.黄鳍鲷不同组织同工酶的研究[J].海洋通报,2004,23(2):92-96. |
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| 作者姓名： | 邓思平 刘楚吾 |
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| 作者单位： | 湛江海洋大学水产学院,广东,湛江,524025 |
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| 基金项目： | 广东省重大科技项目（A200099A0），湛江市科技计划项目（2000 17） |
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| 摘 要： | 运用聚丙烯酰胺垂直梯度凝胶电泳法对黄鳍鲷的7种组织(心肌、肝脏、肾脏、肌肉、性腺、脾脏、脑)进行了4种同工酶(EST，LDH，MDH，ME)的初步研究，并对几种酶的同工酶位点及酶谱表型进行了分析。结果表明：黄鳍鲷的4种同工酶系统具有明显的组织特异性。同时还分析了聚丙烯酰胺垂直梯度凝胶电泳法对同工酶电泳的分离效果，认为该种方法对同工酶系统的分辨率高，分离效果好，可得到更精确的结果。
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| 关 键 词： | 黄鳍鲷 同工酶 组织特异性 |
| 文章编号： | 1001-6392(2004)02-0092-05 |
Isozyme Analysis of Different Tissues in Sparus latus |
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| DENG Siping,LIU Chuwu.Isozyme Analysis of Different Tissues in Sparus latus[J].Marine Science Bulletin,2004,23(2):92-96. |
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| Authors: | DENG Siping LIU Chuwu |
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| Abstract: | By the vertical polyacrylamide gel gradient electrophoresis isozymes (LDH, MDH,ME, EST) from seven tissues (brain, heart, muscle, kidney, gonad, liver, spleen) of Sparus latus
are studied, and the loci and phenotypes are also analyzed. The results show that all isozymes present tissue specificity. The effects of vertical polyacrylamide gel gradient electrophoresis are also analyzed, it is suggested that high distinguishing rate, good effect and precise results can be got to analyze the isozymes system. |
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| Keywords: | Sparus latus Isozyme Tissue specificity |
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