| 微塑料和芘对菲律宾蛤仔的毒性效应研究 |
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| 引用本文： | 柳佳佳,朱效鹏,滕佳,赵建民,李成华,单恩翠,张晨,王清.微塑料和芘对菲律宾蛤仔的毒性效应研究[J].海洋通报,2021,40(6):644-656. |
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| 作者姓名： | 柳佳佳 朱效鹏 滕佳 赵建民 李成华 单恩翠 张晨 王清 |
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| 作者单位： | 宁波大学,浙江宁波315211;中国科学院烟台海岸带研究所海岸带生物资源高教利用研究与发展中心,山东烟台264003;中国科学院烟台海岸带研究所牟平海岸带环境综合试验站,山东烟台264117;中国科学院烟台海岸带研究所海岸带生物资源高教利用研究与发展中心,山东烟台264003;中国科学院大学,北京100049;中国科学院烟台海岸带研究所海岸带生物资源高教利用研究与发展中心,山东烟台264003;中国科学院烟台海岸带研究所牟平海岸带环境综合试验站,山东烟台264117;中国科学院大学,北京100049;宁波大学,浙江宁波315211 |
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| 基金项目： | 国家自然科学基金 （41576122） |
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| 摘 要： | 海洋环境中微塑料和多环芳烃(PAHs)污染日益严重,以滤食动物菲律宾蛤仔(Ruditapes philippinarum)为研究对象,探讨了聚苯乙烯微塑料和芘单一及联合暴露对菲律宾蛤仔的毒性效应.分别采用两个聚苯乙烯微塑料粒径(0.3μm和6μm,20 μg/L)和两个芘浓度水平(10 μg/L和100μg/L)单独和联合暴露21d,测定其对菲律宾蛤仔生理活动(肥满度和摄食率)、免疫防御、氧化应激等相关生物标志物的影响.研究结果表明,除芘单一暴露组外,其余暴露组菲律宾蛤仔的摄食率与对照组相比都显著降低,但这两种污染物对菲律宾蛤仔的肥满度没有显著影响.微塑料和芘暴露均导致菲律宾蛤仔免疫功能受损,表现为血细胞凋亡率增加和吞噬活性被抑制;其中,在小粒径微塑料和高浓度芘存在的暴露组血细胞凋亡率均显著高于对照组,并且二者联合暴露组血细胞凋亡率最高;除大粒径微塑料暴露组外,各暴露组血细胞吞噬活性均显著低于对照组.菲律宾蛤仔抗氧化酶系统不能及时清除体内产生的自由基,导致机体出现氧化损伤现象,表现为抗氧化酶活性变化和丙二醛(MDA)含量升高.微塑料和芘联合暴露导致鳃组织谷胱甘肽疏基转移酶(GST)活性显著升高,消化腺组织过氧化氡酶(CAT)和超氧化物歧化酶(SOD)活性显著降低.综合生物标志物指数(IBR)结果显示,联合暴露对菲律宾蛤仔造成的胁迫压力高于单一暴露组.
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| 关 键 词： | 菲律宾蛤仔 聚苯乙烯微塑料 芘 生理响应 氧化应激 免疫防御 |
| 收稿时间： | 2021/5/11 0:00:00 |
| 修稿时间： | 2021/7/15 0:00:00 |
Toxic effects of polystyrene microplastics and pyrene on Ruditapes philippinarum |
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| LIU Jiajia,ZHU Xiaopeng,TENG Jia,ZHAO Jianmin,LI Chenghua,SHAN Encui,ZHANG Chen,WANG Qing.Toxic effects of polystyrene microplastics and pyrene on Ruditapes philippinarum[J].Marine Science Bulletin,2021,40(6):644-656. |
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| Authors: | LIU Jiajia ZHU Xiaopeng TENG Jia ZHAO Jianmin LI Chenghua SHAN Encui ZHANG Chen WANG Qing |
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| Institution: | Ningbo University, Ningbo 315211, China; Research and Development Center for Efficient Utilization of Coastal Bioresources, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Muping Coastal Environment Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;Research and Development Center for Efficient Utilization of Coastal Bioresources, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Muping Coastal Environment Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China;Research and Development Center for Efficient Utilization of Coastal Bioresources, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | The pollution of microplastics and polycyclic aromatic hydrocarbons (PAHs) in marine environment is increasingly serious. The toxic effects of polystyrene microplastics and pyrene on the clam Ruditapes philippinarum wereinvestigated in this study. R. philippinarum was exposed to two polystyrene microplastics sizes (0.3 um and 6um, 20ug/L) and two pyrene concentrations (10ug/L and 100ug/L) for 21 days, respectively. For individuals in each treatment, physiological responses (condition index and clearance rate), immune defense and oxidative stress parameters were measured. It was found that except for the pyrene single exposure group, the clearance rate of R. philippinarum in the other exposure groups was significantly lower than that of the control group, but the two pollutants had no significant effect on the condition index of R. philippinarum. Exposure to microplastics and pyrene led to impaired immune function of R. philippinarum, manifested by increased hemocyte apoptosis and inhibited phagocytosis. The apoptosis rate of hemocytes in the exposure group with small size microplastics and high concentration pyrene was significantly higher than that in the control group,and the apoptosis rate of hemocytes in the combined exposure group was the highest. Except for the large size microplastic exposure group,the phagocytic activity of hemocytes in each exposure group was significantly lower than that of the control group. The antioxidant system of R. philippinarum could not remove the free radicals produced in the body in time,leading to oxidative damage in the body,which was manifested as the change of antioxidant enzyme activity and the increase of malonic dialdehyde (MDA) content. The combined exposure of microplastics and pyrene significantly increased the activities of glutathione S-transferase (GST) in gill tissues, and significantly decreased the activities of catalase (CAT) and superoxide dismutase (SOD) in digestive gland tissues. Integrated biomarker response (IBR) analysis showed that the stress caused by combined exposure on R. philippinarum was higher than that of the single exposure group. |
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| Keywords: | Ruditapes philippinarum polystyrene microplastics pyrene physiological responses oxidative stress immune defense |
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