| 两种大眼蟹线粒体16S rRNA基因序列分析 |
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| 引用本文： | 徐敬明.两种大眼蟹线粒体16S rRNA基因序列分析[J].海洋通报,2009,28(6). |
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| 作者姓名： | 徐敬明 |
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| 作者单位： | 重庆文理学院,生命科学与技术学院,重庆,永川,402168 |
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| 基金项目： | 重庆文理学院引进人才专项 |
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| 摘 要： | 测定了宽身大眼蟹和日本大眼蟹线粒体16SrRNA基因部分片段的序列,其长度均为517bp。二者的核苷酸序列A,T,G,C的含量相似:宽身大眼蟹分别为33.3%,35.6%,19.9%,11.2%;日本大眼蟹分别为35.2%,35.4%,18.4%,11.0%。不包括6处插入/缺失位点,两序列间有63个变异位点,核苷酸差异率为12.26%,其中转换32个、颠换31个,转换与颠换比约为1.0。对国内外大眼蟹的24条长度为415bp的16SrRNA基因同源序列进行分析,A+T的平均含量为71.7%,明显高于G+C的平均含量,且存有变异位点171个,简约信息位点137个。中国和日本的日本大眼蟹之间的核苷酸差异率为4.42%,表明二者已有明显的遗传分化;中国的日本大眼蟹与日本的万岁大眼蟹之间的核苷酸差异率仅为0.21%,表明二者有可能为同一物种。上述结果得到了系统发生树拓扑结构的支持。
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| 关 键 词： | 宽身大眼蟹 日本大眼蟹 16S rRNA 序列 系统发生 |
Sequence analyses of mitochondrial 16S rRNA gene between two species of macrophthalmid crabs |
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| XU Jing-ming.Sequence analyses of mitochondrial 16S rRNA gene between two species of macrophthalmid crabs[J].Marine Science Bulletin,2009,28(6). |
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| Authors: | XU Jing-ming |
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| Institution: | College of Life Science and Technology;Chongqing University of Arts and Sciences;Yongchuan 402168;Chongqing;China |
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| Abstract: | Partial sequences of the mitochondrial 16S rRNA gene were determined for two Macrophthalmus species (M. dilatatum and M. japonicus),and the sequence length of the two species was the same. Of the 517 nucleotides obtained,the A,T,G and C contents were similar,which were 33.3%,35.6%,19.9%,11.2% and 35.2%,35.4%,18.4%,11.0% respectively. There were 63 different sites (excluding 6 deletion/insertion sites) between the two species,including 32 transition sites and 31 transversion sites. The si/sv and ratio of seq... |
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| Keywords: | Macrophthalmus dilatatum M japonica 16 S rRNA sequence phylogeny |
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