| 几种海水贝类甲醛浸渍标本DNA的提取及rRNA基因ITS序列的扩增 |
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| 引用本文： | 孙博,张素萍,王海艳,刘晓,张国范.几种海水贝类甲醛浸渍标本DNA的提取及rRNA基因ITS序列的扩增[J].海洋通报,2004,23(1):40-44. |
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| 作者姓名： | 孙博 张素萍 王海艳 刘晓 张国范 |
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| 作者单位： | 1. 中国科学院海洋研究所,山东,青岛,266071;中国科学院研究生院，北京 100035
2. 中国科学院海洋研究所,山东,青岛,266071 |
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| 基金项目： | 国家 863 项目资助，课题号：2001AA6201070 |
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| 摘 要： | 从中国科学院海洋生物标本馆收藏的8种甲醛浸渍的海水贝类标本中提取DNA并对其rRNA编码基因的ITS序列进行特异性扩增，在牡蛎Crassostrea sp．、长肋日月贝Amussium pleurondectes (Linnaeus)、扇贝Volachlamys ringaporinug(Sowerby)与华贵栉孔扇贝Chlamys nobilis(Reeve)等4个物种的标本中成功获得扩增产物。利用图片分析软什得出牡蛎标本的ITS-1和ITS-2扩增片段分别为408bp和524bp，长肋日月贝标本分别521bp和537bp，而扇贝Volachlamys ringaporinug(Sowerby)与华贵栉孔扇贝标本只获得ITS-1扩增片段，长度均为545bp。
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| 关 键 词： | 贝类 甲醛浸渍标本 内转录间隔区 牡蛎 日月贝 扇贝 |
| 文章编号： | 1001-6392(2004)01-0040-05 |
DNA Extraction from Marine Shellfish Samples Preserved in Formalin and PCR Amplification of the ITS Region of rRNA Genes |
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| SUN Bo,ZHANG Suping,WANG Haiyan,LIU Xiao,ZHANG Guofan.DNA Extraction from Marine Shellfish Samples Preserved in Formalin and PCR Amplification of the ITS Region of rRNA Genes[J].Marine Science Bulletin,2004,23(1):40-44. |
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| Authors: | SUN Bo ZHANG Suping WANG Haiyan LIU Xiao ZHANG Guofan |
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| Institution: | SUN Bo1,2,ZHANG Suping1,WANG Haiyan1,LIU Xiao1,ZHANG Guofan1 |
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| Abstract: | DNA is extracted successfully from some marine shellfish samples preserved in formalin, which are all taken from the Marine Biological Museum, Chinese Academy of Sciences. PCR amplification for the ITS regions of rRNA genes is achieved successfully in the samples of oyster, scallops Amussium pleurondectes (Linnaeus), Volachlamys ringaporinug (Sowerby) and Chlamys nobilis (Reeve). By using the image processing software, information is obtained as below: the lengths of ITS-1 and ITS-2 of the oysters are 408 bp and 524 bp, respectively; the lengths of ITS-1 and ITS-2 of Amussium pleurondectes (Linnaeus) are 521 bp and 537 bp, respectively. We only get ITS-1 amplification products from the samples of Volachlamys ringaporinug (Sowerby) and Chlamys nobilis (Reeve), which are both 545 bp. |
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| Keywords: | shellfish formalin sample ITS oyster scallop |
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