| 黄鳍鲷消化道内分泌细胞的免疫组化定位 |
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| 引用本文： | 杨青,叶海辉,黄辉洋,韩师昭,王琼.黄鳍鲷消化道内分泌细胞的免疫组化定位[J].海洋通报,2005,24(4):87-90. |
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| 作者姓名： | 杨青 叶海辉 黄辉洋 韩师昭 王琼 |
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| 作者单位： | 厦门大学海洋系,福建,厦门,361005 |
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| 摘 要： | 应用4种兔抗胃肠激素抗体和SABC免疫组织化学方法，对黄鳍鲷（Sparus latus）消化道中的内分泌细胞进行鉴别和定位。结果表明：PP细胞在幽门盲囊较为丰富，食道和小肠较少。Som细胞在贲门胃分布密度最高，在食道和幽门胃也有较多分布。5-HT细胞主要分布在食道和胃部，在幽门盲囊和小肠数量较少。NPY细胞在消化道各部位均未发现。黄鳍鲷消化道PP、Som和5-HT细胞的数量分布和形态特征存在一定的差异，为参与不同的消化生理作用提供了形态学依据。
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| 关 键 词： | 黄鳍鲷 消化道 内分泌细胞 免疫组化 |
| 文章编号： | 1001-6392（2005）04-0087-04 |
| 收稿时间： | 20040811 |
| 修稿时间： | 20040811 |
Immunohistochemical Studies on Endocrine Cells in the Digestive Tract of Sparus latus |
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| YANG Qing,YE Haihui,HUANG Huiyang,HAN Shizhao,WANG Qiong.Immunohistochemical Studies on Endocrine Cells in the Digestive Tract of Sparus latus[J].Marine Science Bulletin,2005,24(4):87-90. |
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| Authors: | YANG Qing YE Haihui HUANG Huiyang HAN Shizhao WANG Qiong |
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| Abstract: | By using the immunohistochemistry method of Strept Avidin-Biotin-Complex, four kinds of antisera raised against rabbit hormones: somatostatin ( Som ), pancreatic polypeptide ( PP ), 5-hydroxytryptamine ( 5-HT ) and neuropeptide Y ( NPY ) were used to indentify and locate the endocrine cells in the digestive tract of S parus latus.T he results reveal that the former three antisera show immunoreactivity. Som-immunoreactive cells are distributed with a highest density in stomachus carsiacus, and a higher density in the esophagus and the stomachus pyloricus. PP-immunoreactive cells were mainly located in the pyloricus sac, while sparsely in the esophagus and the small intestine. 5-HT-immunoreactive cells were found mainly in the esophagus and the stomach, with a few pyloricus sac and the small intestine. No NPY-immunoreactive cells were detected in the in the digestive tract of Sparus latus. The characteristic and density of the 3 kinds of endocrine cells in the digestive tract provided morphological evidence for the digestive physiology of S parus latus. |
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| Keywords: | Sparus latus Digestive tract Endocrine cell Immunohistochemistry |
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