| 双酚A暴露对不同黑色素含量长牡蛎的影响研究 |
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| 引用本文： | 姜秋云,魏磊,王晓娜,陈曼,张镇,孙同振,于文超,何成,宋宏策,李玲玲,王晓通.双酚A暴露对不同黑色素含量长牡蛎的影响研究[J].海洋通报,2019,38(3). |
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| 作者姓名： | 姜秋云 魏磊 王晓娜 陈曼 张镇 孙同振 于文超 何成 宋宏策 李玲玲 王晓通 |
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| 作者单位： | 上海海洋大学 水产科学国家级实验教学示范中心,上海 201306;上海海洋大学 上海水产养殖工程技术研究中心,上海 201306;上海海洋大学水产动物遗传育种中心上海市协同创新水产动物遗传育种中心,上海 201306;鲁东大学 农学院,山东 烟台,264025;鲁东大学 农学院,山东 烟台 264025;上海海洋大学 水产科学国家级实验教学示范中心,上海 201306 |
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| 基金项目： | 国家重点研发计划 （2018YFD0901400）；国家自然科学基金 （41876193）；山东省重点研发计划 （2018GHY115027）；泰山学者专项基金 （tsqn201812094）；山东省自然科学基金 （ZR2017BC058）；山东省高等学校科研计划项目 （J17KA129）；山东省现代农业产业技术体系项目 （SDAIT-14-03）；烟台市重点研发计划 （2017ZH054）；山东省海洋与渔业科技创新计划项目 （2017YY03）。 |
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| 摘 要： | 长牡蛎的壳色有很多种,其中壳色较深个体(黑壳)比较浅个体(白壳)的外套膜中含有更多的黑色素。本研究采用高效液相色谱-质谱法(HPLC-MS/MS)测定天然海水中白壳与黑壳长牡蛎外套膜双酚A (BPA)含量,发现黑壳长牡蛎外套膜中的BPA含量较白壳长牡蛎低(P0.05)。由于黑色素合成过程中的关键——酶酪氨酸酶是一种酚氧化酶,可以通过对氧化酚类或芳胺类等多种底物的氧化起催化作用,推测其在BPA等酚类毒物的降解中也发挥重要作用。为了进一步研究不同黑色素含量牡蛎对BPA降解能力和对BPA损伤抵抗能力的差异,本实验进行了浓度为1 mg/L,1.5 mg/L和2 mg/L的BPA亚慢性暴露实验:通过对暴露后长牡蛎外套膜组织中BPA含量分析发现,白壳与黑壳长牡蛎外套膜中BPA含量均随着暴露浓度的升高而升高,且3种浓度BPA亚慢性暴露后,黑壳长牡蛎外套膜BPA含量均低于白壳长牡蛎,但是差异不显著(P0.05)。采用3种浓度BPA亚慢性暴露后,黑壳长牡蛎外套膜组织中活性氧(ROS)荧光值均显著低于白壳长牡蛎(P0.01)。通过对外套膜组织学观察发现,2 mg/L的高浓度BPA暴露使白壳长牡蛎外套膜组织发生了明显的病理变化,黑壳长牡蛎外套膜未发生明显的病理变化。可见,黑壳长牡蛎对BPA暴露可能具有更强的抵抗能力。
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| 关 键 词： | 长牡蛎 黑色素 酪氨酸酶 双酚A 外套膜 |
| 收稿时间： | 2018/11/6 0:00:00 |
| 修稿时间： | 2018/12/14 0:00:00 |
Effects of bisphenol A exposure on Pacific oyster Crassostrea gigas with different melanin content |
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| JIANG Qiu-yun,WEI Lei,WANG Xiao-n,CHEN Man,ZHANG Zhen,SUN Tong-zhen,YU Wen-chao,HE Cheng,SONG Hong-ce,LI Ling-ling and WANG Xiao-tong.Effects of bisphenol A exposure on Pacific oyster Crassostrea gigas with different melanin content[J].Marine Science Bulletin,2019,38(3). |
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| Authors: | JIANG Qiu-yun WEI Lei WANG Xiao-n CHEN Man ZHANG Zhen SUN Tong-zhen YU Wen-chao HE Cheng SONG Hong-ce LI Ling-ling and WANG Xiao-tong |
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| Institution: | National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China,School of Agriculture, Ludong University, Yantai, 264025, China,School of Agriculture, Ludong University, Yantai, 264025, China,School of Agriculture, Ludong University, Yantai, 264025, China,School of Agriculture, Ludong University, Yantai, 264025, China,School of Agriculture, Ludong University, Yantai, 264025, China,School of Agriculture, Ludong University, Yantai, 264025, China,School of Agriculture, Ludong University, Yantai, 264025, China,School of Agriculture, Ludong University, Yantai, 264025, China,School of Agriculture, Ludong University, Yantai, 264025, China and National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; School of Agriculture, Ludong University, Yantai, 264025, China |
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| Abstract: | There are many variations in the shell color of Pacific oyster Crassostrea gigas, in which darker individuals (black shell) contains more melanin in the mantle than lighter individuals (white shell). In this study, high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) was used to determine the content of bisphenol A (BPA) in the mantle of white shell and black shell oyster in natural seawater. It was found that the content of BPA in the mantle of black shell oyster was lower than that of white shell oyster (P<0.05). Since tyrosinase, a key enzyme in the synthesis of melanin, is a phenol oxidase, which can catalyze the oxidation of phenols or aromatic amines and other substrates, it is speculated that tyrosinase also plays an important role in the degradation of phenolic toxicants such as BPA. To further investigate the differences in the ability of BPA degradation and damage resistance between oysters with different melanin contents, subchronic exposure experiments of BPA with concentrations of 1 mg/L, 1.5 mg/L and 2 mg/L were performed in this study. Through the analysis of the BPA content in the mantle tissues of Crassostrea gigas after exposure, it was found that the BPA content in mantle of white and black shell oyster both increased with the rise of exposure concentration. In addition, after
chronic exposure to BPA at the three concentrations, the BPA content in mantle of black shell oyster was lower than that of white shell oyster, but the difference was not significant (P>0.05). The fluorescence value of reactive oxygen species (ROS) in mantle of black shell oyster was significantly lower than that of white shell oyster after chronic exposure to BPA at three concentrations (P<0.01). The histological observation of the mantle revealed that BPA exposure caused obvious pathological
changes in the mantle tissue of the white shell oyster, while there was no obvious pathological change in the mantle of the black shell oyster, suggesting that the black shell oyster may have stronger resistance to BPA exposure. |
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| Keywords: | Pacific oyster Crassostrea gigas melanin tyrosinase bisphenol A mantle |
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