| 中国明对虾混合养殖池塘沉积物微生物群落比较研究 |
| |
| 引用本文： | 关晓燕,王摆,蒋经伟,田甲申,董颖,陈仲,高杉,王旭达,孙冰,段萍,赵泽龙,周遵春.中国明对虾混合养殖池塘沉积物微生物群落比较研究[J].海洋通报,2020,39(6):730-739. |
| |
| 作者姓名： | 关晓燕 王摆 蒋经伟 田甲申 董颖 陈仲 高杉 王旭达 孙冰 段萍 赵泽龙 周遵春 |
| |
| 作者单位： | 大连海事大学环境科学与工程学院,辽宁大连116023 ;辽宁省农业科学院海洋水产科学研究院辽宁省海洋水产分子生物学重点实验室,辽宁大连116023;辽宁省农业科学院海洋水产科学研究院辽宁省海洋水产分子生物学重点实验室,辽宁大连116023;大连海事大学环境科学与工程学院,辽宁大连116023 |
| |
| 基金项目： | 国家重点研发计划 （2018YFD0901604）；辽宁省“兴辽英才计划” （XLYC1802091）；辽宁省海洋与渔业厅科研项目 （201833； 201830） |
| |
| 摘 要： | 微生物群落在养殖池塘生态系统中发挥着重要的作用，与环境质量及养殖动物的生长和疾病暴发密切相关。为了考察中国明对虾不同混养模式中的微生态状况，本研究基于 16S rRNA 基因的高通量测序技术，比较了 6 种不同中国明对虾混
养池塘沉积物微生物群落的差异。结果表明，6 种混养池塘沉积物的主要微生物均为变形菌门 （Proteobacteria） 和放线菌门（Actinobacteria），与褐牙鲆的混养，会导致变形菌门丰度升高，同时抑制放线菌门的生长。中国明对虾、海蜇、菲律宾蛤仔混养具有最高的沉积物微生物群落丰富度和多样性。通过对微生物功能进行预测发现，化能异养和含硫化合物的呼吸是中国对虾混养池塘沉积物微生物群落的主要功能。中国明对虾、海蜇、菲律宾蛤仔混养沉积物中部分细菌的某特异性富集，可以提高沉积物中溶解氧量并促进化学物污染物的降解。综上所述，中国明对虾、海蜇、菲律宾蛤仔混养模式在 6 种不同中国明对虾混养池塘中微生态状况最佳。
|
| 关 键 词： | 中国明对虾 混合养殖 微生物群落 高通量测序 |
| 收稿时间： | 2020/7/22 0:00:00 |
| 修稿时间： | 2020/9/24 0:00:00 |
Comparisons of sediment microbial communities among six different Penaeus chinensis polyculture ponds |
| |
| GUAN Xiaoyan,WANG Bai,JIANG Jingwei,TIAN Jiashen,DONG Ying,CHEN Zhong,GAO Shan,WANG Xud,SUN Bing,DUAN Ping,ZHAO Zelong,ZHOU Zunchun.Comparisons of sediment microbial communities among six different Penaeus chinensis polyculture ponds[J].Marine Science Bulletin,2020,39(6):730-739. |
| |
| Authors: | GUAN Xiaoyan WANG Bai JIANG Jingwei TIAN Jiashen DONG Ying CHEN Zhong GAO Shan WANG Xud SUN Bing DUAN Ping ZHAO Zelong ZHOU Zunchun |
| |
| Institution: | College of Environment Science and Engineering, Dalian Maritime University, Dalian 116026, China; Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China |
| |
| Abstract: | The microbial community plays an important role in the ecosystem of aquaculture ponds, and is closely related to the environmental quality, the growth of farmed animals and disease outbreaks. In this study, the differences in the sediment microbial communities of six different Penaeus chinensis polyculture ponds were compared based on the 16S rRNA highthroughput sequencing technology to investigate the micro-ecological status in different polyculture modes of Penaeus chinensis. The results showed that the dominant phylum in the sediments of the six polyculture ponds were the Proteobacteria and A ctinobacteria. Co-culture with olive flounder can increase the abundance of Proteobacteria and inhibit the growth of A ctinobacteria. The polyculture of Penaeus chinensis, jellyfish, and clam clams has the highest sediment microbial community richness and diversity. Through the microbial function prediction, it is found that chemical heterotrophic and the sulfurcontaining compounds respiration are the main functions of the microbial community in sediments of Chinese shrimp polyculture ponds. The specific enrichment of some bacteria in the polyculture sediments of Penaeus chinensis, jellyfish and clam clams can increase the amount of dissolved oxygen in the sediments and promote the pollutants degradation. In summary, the polyculture model of Penaeus chinensis, jellyfish, and clam clams has the best micro-ecological status in 6 different polyculture ponds. |
| |
| Keywords: | Fenneropenaeus chinensis aquaculture polyculture microbial community high-throughput sequencing |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《海洋通报》浏览原始摘要信息 |
| 点击此处可从《海洋通报》下载免费的PDF全文 |
|
