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文蛤、青蛤和四角蛤蜊的随机扩增多态性DNA(RAPD)的比较分析
引用本文:陈大鹏,沈怀舜,丁亚平,杨家新,许璞.文蛤、青蛤和四角蛤蜊的随机扩增多态性DNA(RAPD)的比较分析[J].海洋通报,2004,23(6):84-87.
作者姓名:陈大鹏  沈怀舜  丁亚平  杨家新  许璞
作者单位:1. 南京师范大学生命科学院,江苏,南京,210097
2. 江苏省海洋与水产研究所,江苏,南通,226007
摘    要:利用随机扩增多态性DNA(RAPD)技术,对吕四海区的三种重要的水产贝类:文蛤、青蛤和四角蛤蜊的遗传多样性进行了分析和比较。用60个随机引物对三种贝类共24个样品进行随机扩增,从中选取了45个扩增效果比较稳定的引物用于群体分析,共得到了250个位点。根据Nei氏公式计算出3种贝类种间和种内的遗传相似性指数和相对遗传距离,并进行聚类分析。通过比较分析,3种贝类之间的遗传距离差异不大。相对与青蛤和四角蛤蜊,文蛤种内的遗传距离要小。

关 键 词:随机扩增多态性DNA  基因组DNA  四角蛤蜊  青蛤  文蛤
文章编号:1001-6392(2004)06-0084-04

Randomly Amplified Polymorphic DNA Analysis of Meretrix meretrix,Cyclina sinesis and Mactra vecerifermis
CHEN Dapeng,SHEN Huaishun,DING Yaping,YANG Jiaxin,XU Pu.Randomly Amplified Polymorphic DNA Analysis of Meretrix meretrix,Cyclina sinesis and Mactra vecerifermis[J].Marine Science Bulletin,2004,23(6):84-87.
Authors:CHEN Dapeng  SHEN Huaishun  DING Yaping  YANG Jiaxin  XU Pu
Institution:CHEN Dapeng,SHEN Huaishun,DING Yaping,YANG Jiaxin,XU Pu 1 2 2 1 2
Abstract:Meretrix meretrix, Cyclina sinesis and Mactra vecerifermis are important aquaculture shellfish in Jiangsu Province. The genetic polymorphisms of these shellfishes, collected from the Lusi coastal area, were studied by the randomly amplified polymorphic DNA technique. Sixty random primers were used to test the 24 samples of three kinds of shellfishes. Forty-five primers were chosen for generating a total of 250 highly steady bands. According to Nei's formula, the genetic comparability index was counted. A phylogenetic tree was constructed using the UPGMA program based on the indexes of three species of shellfishes. The quantities of Meretrix meretrix's germ plasm have been declining through studies of comparing genetic polymorphisms of these three shellfishes.
Keywords:RAPD  Genomic DNA  shellfish  Meretrix meretrix  Cyclina sinensis  Mactra  veneriformis
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