| fSSR分析技术的建立及在大黄鱼(Larimichthys crocea)亲子鉴定中的应用 |
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| 引用本文： | 武祥伟,刘贤德,王志勇.fSSR分析技术的建立及在大黄鱼(Larimichthys crocea)亲子鉴定中的应用[J].海洋通报,2011,30(4):419-424. |
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| 作者姓名： | 武祥伟 刘贤德 王志勇 |
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| 作者单位： | 集美大学 水产学院 农业部东海海水健康养殖重点实验室,福建厦门,361021 |
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| 基金项目： | 公益性行业（农业）科研专项(200903029-4)；国家自然科学基金项目(30771663)；集美大学优秀青年骨干教师基金（2009C002） |
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| 摘 要： | 以大黄鱼(Larimichthys crocea)为材料,对荧光标记微卫星(fSSR)分析体系进行了优化,建立了适合大黄鱼fSSR分析的最佳条件.与常规的银染法相比,该方法成本略高,但其分辨率高,可重复性强,检测效率为银染法的6～10倍.利用优化的fSSR 技术进行了大黄鱼亲于鉴定的研究,结果显示在使用6对引物的情况下...
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| 关 键 词： | fSSR 大黄鱼 亲子鉴定 Gel-Scan 3000 |
| 收稿时间： | 2010/11/25 0:00:00 |
Condition exploration and optimization for fSSR system and its application in large yellow croaker Larimichthys crocea for parentage assignment |
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| WU Xiang-wei,LIU Xian-de and WANG Zhi-yong.Condition exploration and optimization for fSSR system and its application in large yellow croaker Larimichthys crocea for parentage assignment[J].Marine Science Bulletin,2011,30(4):419-424. |
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| Authors: | WU Xiang-wei LIU Xian-de and WANG Zhi-yong |
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| Institution: | Key Laboratory of Healthy Mariculture for East China Sea, Ministry of Agriculture of China, Fisheries College, Jimei University, Xiamen 361021, China;Key Laboratory of Healthy Mariculture for East China Sea, Ministry of Agriculture of China, Fisheries College, Jimei University, Xiamen 361021, China;Key Laboratory of Healthy Mariculture for East China Sea, Ministry of Agriculture of China, Fisheries College, Jimei University, Xiamen 361021, China |
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| Abstract: | In this study, the fluorescent labeling microsatellite (fSSR) analysis system was constructed and optimized in larger yellow croaker Larimichthys crocea. Although the cost of the fluorescent method was a little higher than that of the silver-staining method, it was 6~10 times efficient compared with the latter with high resolution and repetition . Furthermore, the parentage assignment rates for two groups of large yellow croaker were both more than 99% using optimized fSSR with six microsatellite loci, which means this technology is very usefull. |
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| Keywords: | fSSR Larimichthys crocea paretage assignment Gel-Scan 3000 |
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