| 自养小球藻（Chlorella autotrophica）多糖体外抑菌活性的初步研究 |
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| 引用本文： | 刘四光,李文权,黄智伟,陈岚,王键,张钒.自养小球藻（Chlorella autotrophica）多糖体外抑菌活性的初步研究[J].海洋通报,2012,31(1):45-50. |
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| 作者姓名： | 刘四光 李文权 黄智伟 陈岚 王键 张钒 |
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| 作者单位： | 1. 福建海洋研究所福建省海陆界面生态环境联合重点实验室,福建厦门,361013
2. 厦门大学海洋系亚热带海洋研究所,福建厦门,361005 |
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| 基金项目： | 国家自然科学基金（39870565）和厦门大学科研基金（20005003） |
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| 摘 要： | 选取了自养小球藻(Chlorella autotrophica)为研究对象,以超声辅助提取法进行硫酸多糖提取,所得粗多糖采用凝胶法在AKTA系统下进行分离纯化得到PCA2-1及PCA2-2共2种硫酸多糖,电泳及HPGFC法检验结果表明所得硫酸多糖的均为均一多糖。选取了4种在海洋环境或医学中常见的菌株为作为受试菌株,分别进行了PCA2-1和PCA2-2抑菌活性的初步实验,其中大肠杆菌抗生素检定株(Escherichia coli)和解藻朊酸弧菌(Vibrio alginolyticus)为革兰氏阴性菌,表皮葡萄球菌(Staphytococcus epidermidis)和溶壁微球菌(Micrococcus lysodeikticus)为革兰氏阳性菌。所得结果表明,这2种多糖均对某种特定的菌株表现出一定的抑制作用。其中,PCA2-2对溶壁微球菌和解藻朊酸弧菌有较强有抑菌作用,KI值可达70%以上。
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| 关 键 词： | 硫酸多糖 自养小球藻 抑菌活性 |
| 收稿时间： | 2011/4/18 0:00:00 |
| 修稿时间： | 2011/8/12 0:00:00 |
Preliminary study on in vitro antibacterial activity of polysaccharides from Chlorella autotrophica |
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| LIU Siguang,LI Wenquan,HUANG Zhiwei,CHEN Lan,WANG Jian and ZHANG Fan.Preliminary study on in vitro antibacterial activity of polysaccharides from Chlorella autotrophica[J].Marine Science Bulletin,2012,31(1):45-50. |
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| Authors: | LIU Siguang LI Wenquan HUANG Zhiwei CHEN Lan WANG Jian and ZHANG Fan |
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| Institution: | Fujian Institute of Oceanology, Xiamen, 361013, China;Department of Oceanography, Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China;Fujian Institute of Oceanology, Xiamen, 361013, China;Fujian Institute of Oceanology, Xiamen, 361013, China;Fujian Institute of Oceanology, Xiamen, 361013, China;Fujian Institute of Oceanology, Xiamen, 361013, China |
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| Abstract: | Polysaccharides from Chlorella autotrophica are extracted with ultrasonic assistant method in the study. AKTA purifier system is applied in the isolation, and we obtain two polysaccharides, PCA2-1 and PCA2-2, both of which are verified to be homologous by electrophoresis and HPGFC. Antibacterial experiments of PCA2-1 and PCA2-2 are carried out and the bacteria that are common in marine environment and medicine are selected, including Escherichia coli(G-), Vibrio alginolyticus(G-), Staphylococcus epidermidis(G+) and Micrococcus lysodeikticus(G+). Results show that the two polysaccharides can inhibit some specific bacterium to some extent. PCA2-2 has a steady and intensive effect on Micrococcus lysodeikticus and Vibrio alginolyticus and the killing index (KI) exceeds 70%. |
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| Keywords: | sulfated polysaccharide Chlorella autotrophica antibacterial activity |
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