| 不同培养条件对三角褐指藻生长及其生物活性成分积累的影响 |
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| 引用本文： | 张文源,高保燕,李爱芬,张成武.不同培养条件对三角褐指藻生长及其生物活性成分积累的影响[J].海洋科学,2016,40(5):57-65. |
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| 作者姓名： | 张文源 高保燕 李爱芬 张成武 |
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| 作者单位： | 暨南大学 水生生物研究中心生态学系,暨南大学 水生生物研究中心生态学系,暨南大学 水生生物研究中心生态学系,暨南大学 水生生物研究中心生态学系 |
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| 基金项目： | 国家“863”项目(2013AA065805); 国家自然科学基金项目(31170337); 广东省低碳专项(2011-051); 珠海市科技重大项目(PB20041018); 珠海市科技攻关项目(PC20081008) |
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| 摘 要： | 以三角褐指藻(Phaeodactylum tricornutum)为研究材料,比较了Na NO3、NH4HCO3和CO(NH2)2为氮源的两种培养基(m L1和ASW培养基)对其生长和生物活性成分(岩藻黄素、金藻昆布糖和二十碳五烯酸(C20:5,EPA))时相积累的影响,同时分析了脂肪酸组成和总脂含量的变化。结果表明:以m L1培养基培养时,三角褐指藻的生物质质量浓度明显高于ASW培养基培养时的生物质质量质量浓度,尿素优于其他两种氮源,最大生物质质量质量浓度为3.7 g/L。不同培养条件下岩藻黄素含量的时相变化规律一致,均随着培养时间的延长呈现先增加后减少的趋势,其最高积累量分别为:13.27 mg/g(Na NO3)、13.23 mg/g(CO(NH2)2)和13.89 mg/g(NH4HCO3)(m L1);13.2 mg/g(Na NO3)、14.92 mg/g(CO(NH2)2)和13.6 mg/g(NH4HCO3)(ASW),由此可知氮源对岩藻黄素积累量影响不大。金藻昆布糖含量随着培养时间延长逐渐增加,其最大积累量分别为9.82 mg/g(NH4HCO3)(m L1)和8.59 mg/g(Na NO3)(ASW)。不同培养条件下其总脂含量变化不显著,均在培养末期达到最大值,分别为24.18%(NH4HCO3)(m L1)和23.79%(Na NO3)(ASW);其主要脂肪酸组成为:豆蔻酸(C14:0)、棕榈酸(C16:0)、棕榈油酸(C16:1)、硬脂酸(C18:0)、油酸(C18:1)、亚油酸(C18:2)、花生一烯酸(C20:1)、木焦油酸(C24:0)和EPA,其中,EPA含量随着培养时间延长逐渐下降,尿素最有利于EPA的积累。
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| 关 键 词： | 三角褐指藻 生物质质量浓度 岩藻黄素 金藻昆布糖 二十碳五烯酸 |
| 收稿时间： | 2015/11/26 0:00:00 |
| 修稿时间： | 2/6/2016 12:00:00 AM |
Effects of different culture conditions on growth and accumulation of bioactive compounds by Phaeodactylum tricornutum |
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| ZHANG Wen-yuan,GAO Bao-yan,LI Ai-fen and ZHANG Cheng-wu.Effects of different culture conditions on growth and accumulation of bioactive compounds by Phaeodactylum tricornutum[J].Marine Sciences,2016,40(5):57-65. |
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| Authors: | ZHANG Wen-yuan GAO Bao-yan LI Ai-fen and ZHANG Cheng-wu |
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| Abstract: | In this study, the effects of mL1 and ASW media containing NaNO3, CO(NH2)2, or NH4HCO3 on the growth and accumulation of bioactive compounds fucoxanthin, chrysolaminaran, and eicosapentaenoic acid (EPA)] by the marine diatom Phaeodactylum tricornutum were investigated; and the total lipid content and fatty acid composition were analyzed. The results showed that maximum biomass concentration in mL1 medium with urea as the nitrogen source was 3.7 g/L. Fucoxanthin content initially increased and then decreased during culture under different conditions, and the highest quantities that accumulated were 13.27 mg/g (NaNO3), 13.23 mg/g (CO(NH2)2), and 13.89 mg/g (NH4HCO3) in mL1 medium and 13.2 mg/g (NaNO3), 14.92 mg/g (CO(NH2)2), and 13.6 mg/g (NH4HCO3) in ASW medium, indicating that the nitrogen source had a weak effect on fucoxanthin accumulation. Chrysolaminaran content increased gradually during the culture period, and the highest contents were 9.82 mg/g (NH4HCO3) in mL1 and 8.59 mg/g (NaNO3) in ASW on day 15. The total lipid content changed slightly under the different conditions, and the maximum values were 24.18% (NH4HCO3) in mL1 and 23.79% (NaNO3) in ASW, which were observed during the stationary phase. The fatty acid profiles were myristic acid (C14: 0), palmitic acid (C16: 0), palmitoleic acid (C16: 1), stearic acid (C18: 0), oleic acid (C18: 1), linoleic acid (C18: 2), eicosaenoic acid (C20: 1), tetracosanoic acid (C24: 0), and EPA (C20: 5). The EPA content decreased gradually as culture time was extended. Urea was the optimal nitrogen source to accumulate EPA. |
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| Keywords: | Phaeodactylum tricornutum biomass concentration fucoxanthin chrysolaminaran eicosapentaenoic acid |
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