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| 养殖大菱鲆细菌性红体病病原菌的分离与鉴定 | |
| 引用本文： | 董丽,王印庚,张正,曲江波,陈霞.养殖大菱鲆细菌性红体病病原菌的分离与鉴定[J].海洋科学,2009,33(7):57-63. |
| 作者姓名： | 董丽 王印庚 张正 曲江波 陈霞 |
| 作者单位： | 1. 中国海洋大学,生命学院,山东,青岛,266003;中国水产科学研究院,黄海水产研究所,青岛市海水鱼类种子工程与生物技术重点实验室,山东,青岛,266071 2. 中国水产科学研究院,黄海水产研究所,青岛市海水鱼类种子工程与生物技术重点实验室,山东,青岛,266071 3. 天源水产有限公司,山东,烟台,264000 4. 青岛九洋红水产科技有限公司,山东,青岛,266071 |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家科技支撑计划，公益性行业(农业)科技专项项目 |
| 摘 要： | 从患有红体病的养殖大菱鲆(Scophthalmus maximus)的肌肉中分离得到一株优势菌并记为H1.经人工注射感染证实H1即为引起养殖大菱鲆红体病的病原菌,其半致死量LD50为2.82×105CFU/mL,而低浓度(1.41×103 CFU/mL)没有造成死亡,但注射部位有脓肿现象,注射相同体积1.5%无菌生理盐水的对照组没有明显的症状,注射部位也无异常.革兰氏染色显示该菌为革兰氏阴性,菌体呈杆状,周生鞭毛.综合该菌的形态、常规生理生化特征和API32E鉴定结果,发现H1与迟钝爱德华氏菌的表性特征非常相似,相似率迭到99.9%.在分子水平上对其16SRNA基因序列的测定,同源性分析,结果表明H1与迟钝爱德华氏菌的亲缘关系最近,相似度达到99%.综合上述研究结果,将该菌株初步鉴定为迟钝爱德华氏茵(Edwardsiella tarda). |
| 关 键 词： | 大菱鲆(Scophthalmus maximus) 红体病 细菌鉴定 迟钝爱德华氏菌(Edwardsiella tarda) |
| 收稿时间： | 9/2/2008 12:00:00 AM |
| 修稿时间： | 2008/12/2 0:00:00 |
Isolation and identification of the pathogentic bacterium causing red body from cultured Turbot Scophthalmus maximus |
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| DONG Li,WANG Yin-geng,ZHANG Zheng,QU jiang-bo and CHEN Xia.Isolation and identification of the pathogentic bacterium causing red body from cultured Turbot Scophthalmus maximus[J].Marine Sciences,2009,33(7):57-63. | |
| Authors: | DONG Li WANG Yin-geng ZHANG Zheng QU jiang-bo and CHEN Xia |
| Abstract: | A dominant strain of the bacteria causing red body of turbot was isolated from the diseased turbot and designated as Hl.In an artificial infection test ,all fish of the experimental group died in 15 days after intramuscularly being injected with a high bacterial suspention (1.41×109CFU/mL) , and the low bacterial suspention (1.41 × CFU 103/mL) did not cause any death, while the control group showed no signs all through the experimentation. The LD50 was calculated as 2.82×105CFU/mL.The moribund experimental fish had gross signs similar to the natural infected fish. The bacterium re- isolated from the challenged fish also had the same characteristics as H1, which proved that the isolated H1 was the pathogenic bacterium that triggered this red body disease. Different methods were used to identify the pathogenic bacterium. The bacterium is negative, rod in shape, peritrichous flagella under electronic microscope. Traditional biochemical identification revealed that H1 exhibited relatedness to Edwardsiella tarda, while the identification result by API 32E system indicated that H1 was E. tarda with 99.9% reliability. Its 16S ribosomal RNA gene sequences were analyzed, and it is shown from the aniysis results that this strain of bacterium has a close phylogenetic relation (99%)to E.tarda. In summary, the pathogenic bacterium was identified as E. tarda. |
| Keywords: | Scophthalmus maximus red body identification of bacterium Edwardsiella tarda |
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