| 海参酶解物中抗老年痴呆相关活性及成分初步研究 |
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| 引用本文： | 杨静明,卢虹玉,谢慧风,刘亚月,聂影影,张永平,宋采,秦邦伟,张翼.海参酶解物中抗老年痴呆相关活性及成分初步研究[J].海洋科学,2019,43(7):41-52. |
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| 作者姓名： | 杨静明 卢虹玉 谢慧风 刘亚月 聂影影 张永平 宋采 秦邦伟 张翼 |
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| 作者单位： | 广东海洋大学 食品科技学院, 广东省海洋生物制品工程实验室, 广东省水产品加工与安全重点实验室,广东海洋大学海洋药物研究所, 广东 湛江 524088;广东海洋大学 食品科技学院, 广东省海洋生物制品工程实验室, 广东省水产品加工与安全重点实验室,广东海洋大学海洋药物研究所, 广东 湛江 524088;广东海洋大学 深圳研究院, 广东 深圳 518120;山东圣洲海洋生物科技股份有限公司,山东 威海,264211 |
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| 基金项目： | 广东省扬帆计划引进紧缺拔尖人才项目（201433009）；广东省自然科学基金（2018A030307046）；广东海洋大学科研启动项目（E15155）；深圳市科创委基础研究自由探索项目（JCYJ20170306165013264）；深圳市大鹏新区产业发展资金（KY20180203；PT201901-05；KY20170210） |
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| 摘 要： | 通过国标方法分析海参酶解原液的基本营养成分;并将海参酶解原液用不同极性的有机溶剂进行提取,利用乙酰胆碱酯酶抑制活性自显影的方法进行活性组分的追踪。利用硅胶柱层析、Sephadex LH-20凝胶柱、高效液相色谱逐步纯化,得到具有较高乙酰胆碱酯酶抑制活性的组分;另外,采用MTT法考察海参酶解液总脂溶性组分对小胶质细胞(BV-2细胞)生长的影响;并利用LPS诱导细胞建立细胞炎症模型,分别采用Griess法和ELISA法检测其对LPS诱导的BV-2细胞中NO和IL-6水平的影响。结果表明:海参酶解原液含有丰富的钙、锌等矿物质,含量较高的多不饱和脂肪酸和必需氨基酸其提取物中具有乙酰胆碱酯酶抑制活性的薄层层析斑点均无明显紫外与荧光吸收,其中活性最强组分C4-2-E在浓度为0.25g/L时对乙酰胆碱酯酶的抑制率为91.04%;海参酶解液总脂溶性组分在一定的浓度范围内对BV-2细胞均无显著的抑制作用,并且能够显著的抑制LPS诱导的BV-2细胞所产生的NO和IL-6的分泌。故海参酶解液的脂溶性成分可能具有一定的抗神经炎症和老年痴呆作用,具体活性物质的化学结构还需进一步分析鉴定。
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| 关 键 词： | 海参 提取分离 阿尔兹海默氏症 乙酰胆碱酯酶抑制活性 抗炎活性 |
| 收稿时间： | 2019/1/6 0:00:00 |
| 修稿时间： | 2019/3/22 0:00:00 |
Preliminary study on anti-Alzheimer's activity and composition in sea cucumber hydrolysate |
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| YANG Jing-ming,LU Hong-yu,Xie Hui-feng,LIU Ya-yue,NIE Ying-ying,ZHANG Yong-ping,SONG Cai,QIN Bang-wei and ZHANG Yi.Preliminary study on anti-Alzheimer''s activity and composition in sea cucumber hydrolysate[J].Marine Sciences,2019,43(7):41-52. |
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| Authors: | YANG Jing-ming LU Hong-yu Xie Hui-feng LIU Ya-yue NIE Ying-ying ZHANG Yong-ping SONG Cai QIN Bang-wei and ZHANG Yi |
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| Institution: | College of Food Science and Technology, Guangdong Ocean University, Guangdong Marine Biological Products Engineering Laboratory, Key Laboratory of Guangdong Province Aquatic Products Processing and Safety, Research Institute for Marine Drugs and Nutrition, Guangdong Ocean University, Zhanjiang, 524088, China,College of Food Science and Technology, Guangdong Ocean University, Guangdong Marine Biological Products Engineering Laboratory, Key Laboratory of Guangdong Province Aquatic Products Processing and Safety, Research Institute for Marine Drugs and Nutrition, Guangdong Ocean University, Zhanjiang, 524088, China,College of Food Science and Technology, Guangdong Ocean University, Guangdong Marine Biological Products Engineering Laboratory, Key Laboratory of Guangdong Province Aquatic Products Processing and Safety, Research Institute for Marine Drugs and Nutrition, Guangdong Ocean University, Zhanjiang, 524088, China,College of Food Science and Technology, Guangdong Ocean University, Guangdong Marine Biological Products Engineering Laboratory, Key Laboratory of Guangdong Province Aquatic Products Processing and Safety, Research Institute for Marine Drugs and Nutrition, Guangdong Ocean University, Zhanjiang, 524088, China,College of Food Science and Technology, Guangdong Ocean University, Guangdong Marine Biological Products Engineering Laboratory, Key Laboratory of Guangdong Province Aquatic Products Processing and Safety, Research Institute for Marine Drugs and Nutrition, Guangdong Ocean University, Zhanjiang, 524088, China;Shenzhen Insititute of Guangdong Ocean University, Shenzhen, 518120, China,College of Food Science and Technology, Guangdong Ocean University, Guangdong Marine Biological Products Engineering Laboratory, Key Laboratory of Guangdong Province Aquatic Products Processing and Safety, Research Institute for Marine Drugs and Nutrition, Guangdong Ocean University, Zhanjiang, 524088, China,College of Food Science and Technology, Guangdong Ocean University, Guangdong Marine Biological Products Engineering Laboratory, Key Laboratory of Guangdong Province Aquatic Products Processing and Safety, Research Institute for Marine Drugs and Nutrition, Guangdong Ocean University, Zhanjiang, 524088, China,Shandong Shengzhou Marine Biological Technology Co., Ltd. Weihai, 264211, China and College of Food Science and Technology, Guangdong Ocean University, Guangdong Marine Biological Products Engineering Laboratory, Key Laboratory of Guangdong Province Aquatic Products Processing and Safety, Research Institute for Marine Drugs and Nutrition, Guangdong Ocean University, Zhanjiang, 524088, China;Shenzhen Insititute of Guangdong Ocean University, Shenzhen, 518120, China |
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| Abstract: | |
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| Keywords: | sea cucumber extraction and separation Alzheimer''s disease acetylcholinesterase inhibitory activity anti-inflammatory activity |
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