| 栉孔扇贝(♀)×虾夷扇贝(♂)子一代杂种优势的SRAP分析 |
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| 引用本文： | 程宁宁,杨爱国,刘志鸿,周丽青,吴 彪.栉孔扇贝(♀)×虾夷扇贝(♂)子一代杂种优势的SRAP分析[J].海洋科学,2009,33(10):107-111. |
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| 作者姓名： | 程宁宁 杨爱国 刘志鸿 周丽青 吴 彪 |
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| 作者单位： | 1. 中国水产科学院,黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,山东,青岛266071;上海海洋大学,生命科学与技术学院,上海,200090
2. 中国水产科学院,黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,山东,青岛266071 |
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| 基金项目： | 国家863计划资助项目，国家科技支撑计划资助项目，国家科技基础条件平台建设资助项目 |
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| 摘 要： | 采用新型的分子标记技术(Sequence-related amplified polymorphism,简称SRAP)对栉孔扇贝(Chlamys farreri)(♀)×虾夷扇贝(Patinopecten yessoensis)(♂)子一代及其双亲的遗传变异进行分析.从不同的引物组合中筛选出9对条带清晰、多态性丰富的组合,9对引物共产生了121条扩增带,其中多态性条带107条,平均每对引物组合产生11.89条多态标记.Shannon信息指数I分别为0.299 9、0.218 9、0.334 3,Nei's基因多样性指数h分别是0.196 4、0.141 1、0.218 9,栉孔扇贝与杂交后代的遗传距离是0.107 4,虾夷扇贝与杂交后代的遗传距离是0.228 7,聚类分析也显示了这一特点.从变异贡献率来看,3个群体的总变异中,有37.31%的变异来源于群体间,62.69%的变异来源于个体间.
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| 关 键 词： | 栉孔扇贝(Chlamys farreri) 虾夷扇贝(Patinopecten yessoensis) 杂种优势 |
| 收稿时间： | 2009/6/18 0:00:00 |
| 修稿时间： | 2009/8/11 0:00:00 |
Analysis on heterosis of Chlamys farreri × Patinopecten yes-soensis by SRAP marker |
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| CHENG Ning-ning,YANG Ai-guo,LIU Zhi-hong,ZHOU Li-qing and WU Biao.Analysis on heterosis of Chlamys farreri × Patinopecten yes-soensis by SRAP marker[J].Marine Sciences,2009,33(10):107-111. |
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| Authors: | CHENG Ning-ning YANG Ai-guo LIU Zhi-hong ZHOU Li-qing and WU Biao |
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| Abstract: | Sequence-related amplified polymorphism(SRAP)markers were used to examine genetic varia-tions of two parents Chlamys farreri(♀), Patinopecten yessoensis(♂)and their hybrids(F1). 9 pairs of primer were selected from 64 pairs of primer and applied to 3 populations. A total of 121 bands were genera-ted with 9 pairs of primers sets, among them 107 were polymorphic. In average, each primer set deteced 11.89 polymorphic loci. Shannon information indexes were 0.299 9, 0.218 9, 0.334 3 and Nei's gene diver-sities were 0.196 4, 0.141 1, 0.218 9. The genetic distance between C. Farreri and F1 was 0.107 4, be-tween P. Yessoensis and F1 was 0.228 7. |
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| Keywords: | SRAP Chlamys farreri Patinopecten yessoensis heterosis: SRAP |
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