| 日本蟳血细胞及血液生化指标的研究 |
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| 引用本文： | 许星鸿,阎斌伦,郑家声,邵营泽,徐加涛,徐国成,周 盟,周 黎.日本蟳血细胞及血液生化指标的研究[J].海洋科学,2011,35(8):51-57. |
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| 作者姓名： | 许星鸿 阎斌伦 郑家声 邵营泽 徐加涛 徐国成 周 盟 周 黎 |
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| 作者单位： | 淮海工学院 江苏省海洋生物技术重点建设实验室;淮海工学院 江苏省海洋生物技术重点建设实验室;中国海洋大学海洋生命学院;淮海工学院 江苏省海洋生物技术重点建设实验室;淮海工学院 江苏省海洋生物技术重点建设实验室;淮海工学院 江苏省海洋生物技术重点建设实验室;淮海工学院 江苏省海洋生物技术重点建设实验室;淮海工学院 江苏省海洋生物技术重点建设实验室 |
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| 基金项目： | 江苏省海洋生物技术重点建设实验室开放课题(Z2007HS006); 江苏省科技厅苏北科技发展计划项目(BN2010026) |
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| 摘 要： | 以光镜和电镜观察了日本蝠（Charybdisjaponica）血细胞的形态结构，分析了各类血细胞的大小、数量比例及功能，并测定了不同发育时期及饥饿状态的日本蝽血清主要生化指标。结果表明：日本蝠血细胞可分为4种类型，从小到大依次为：无颗粒细胞、小颗粒细胞、中间型细胞和大颗粒细胞；其核质比分别为：53．23％、33．64％...
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| 关 键 词： | 日本蟳(Charybdis japonica) 血细胞 血液生化指标 吞噬能力 饥饿 |
| 收稿时间： | 2010/6/26 0:00:00 |
| 修稿时间： | 2011/8/21 0:00:00 |
Hemocytes and blood biochemical characterization of Charybdis japonica |
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| XU Xing-hong,YAN Bin-lun,ZHENG Jia-sheng,SHAO Ying-ze,XU Jia-tao,XU Guo-cheng,ZHOU Meng,ZHOU Li.Hemocytes and blood biochemical characterization of Charybdis japonica[J].Marine Sciences,2011,35(8):51-57. |
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| Authors: | XU Xing-hong YAN Bin-lun ZHENG Jia-sheng SHAO Ying-ze XU Jia-tao XU Guo-cheng ZHOU Meng ZHOU Li |
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| Institution: | XU Xing-hong1,YAN Bin-lun1,ZHENG Jia-sheng2,SHAO Ying-ze1,XU Jia-tao1,XU Guo-cheng1,ZHOU Meng1,ZHOU Li1(1.Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China,2.College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | In order to provide data for tissue pathology, disease control, and reproductive physiological research of Charybdis japonica, this paper reported the hemocytes and the blood biochemical characterization of Charybdis japonica. With light and electronic microscopes, four types of hemocytes, including agranular hemocyte (AG), small granular hemocyte (SG), intermediate granular hemocyte (IG) and large granular hemocyte (LG), were classified according to their granular existence, granular size, refractivity, staining characters, and nuclear-cytoplasmic ratio. The nuclear-cytoplasmic ratios were 53.23%(AG), 33.64%(SG), 24.50% (IG) and 19.45% (LG), respectively. Among these hemocytes, the percentages of AG, SG, IG and LG were 4.78%, 42.69%, 36.57%, and 15.96%, respectively. The female crab had higher density of hemocytes than the male, but there was no significant difference in cell size. The phagocytic capacity of hemocytes from strong to weak were IG, LG and SG. AG had no phagocytic capacity. The hemocytes in gill had big pseudopodia and abundant residual body suggesting they could be more active in phagorytosis. In the developing phase, the values of total protein(TP), glucose(GLU) and cholesterin(CHOL) of female were higher than those of male, but the male crabs in maturation had more TP than the female. After spawning, the female crabs could maintain high levels of TP and CHOL. After starvation for seven days, the values of density of hemocytes, TP, GLU, and CHOL decreased obviously and there were lower activities of alanine aminotransferase(ALT) and alkaline phosphatase(ALP) than before, but the levels of creatinine(CREA) and usea nitrogen(BUN) increased. Globin was found in the blood serum of Charybdis japonica. |
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| Keywords: | Charybdis japonica hemocyte blood biochemical value phagocytic capacity starvation |
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