| 海洋枯草芽孢杆菌HS-A38 产直链脂肽活性物质的初步检测 |
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| 引用本文： | 张 欢,丛丽娜,侯英敏,李爱民,谢三群,王 丹.海洋枯草芽孢杆菌HS-A38 产直链脂肽活性物质的初步检测[J].海洋科学,2011,35(8):37-42. |
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| 作者姓名： | 张 欢 丛丽娜 侯英敏 李爱民 谢三群 王 丹 |
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| 作者单位： | 大连工业大学;大连工业大学;大连工业大学;大连工业大学;大连工业大学;大连工业大学 |
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| 基金项目： | 国家自然科学基金项目(31072224) , 辽宁省教育厅创新团队资助项目(LT2010012); 辽宁省自然科学基金项目(20102009) |
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| 摘 要： | 从海参肠道中分离得到了一株海洋枯草芽孢杆菌(Bacillus subtilis)HS-A38, 将其发酵上清液经盐酸沉淀、有机溶剂萃取得到具有广谱抗菌活性的代谢物质。采用薄层层析和茚三酮显色检测到4 种物质, 分别命名为化合物1、2、3 和4; 应用层析板覆盖指示菌的生物自显影技术对其抗菌活性成分进行追踪、检测, 结果发现化合物1 和2 具有显著抑制弧菌的作用。进一步检测分析表明, 化合物1、2和4 具有很强的热稳定性, 初步推断它们属于直链的脂肽类化合物, 并且这4 种物质出现在不同的发酵代谢时期, 这将为研究群体效应提供基础检测依据。
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| 关 键 词： | 枯草芽孢杆菌(Bacillus subtilis) 抗菌活性 生物自显影 直链脂肽化合物 |
| 收稿时间： | 2010/10/11 0:00:00 |
| 修稿时间： | 3/2/2011 12:00:00 AM |
Antimicrobial activity of liner lipopeptides produced by Bacillus subtilis HS-A38 |
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| ZHANG Huan,CONG Li-na,HOU Ying-min,LI Ai-min,XIE San-qun,WANG Dan.Antimicrobial activity of liner lipopeptides produced by Bacillus subtilis HS-A38[J].Marine Sciences,2011,35(8):37-42. |
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| Authors: | ZHANG Huan CONG Li-na HOU Ying-min LI Ai-min XIE San-qun WANG Dan |
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| Institution: | ZHANG Huan,CONG Li-na,HOU Ying-min,LI Ai-min,XIE San-qun,WANG Dan(Dalian Polytechnic University,Dalian 116034,China) |
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| Abstract: | A marine Bacillus subtilis strain HS-A38 was isolated from the sea cucumber intestine. The crude extract of fermentation broth was prepared by methods of hydrochloric acid precipitation and solvent extraction. The extract showed broad-spectrum antimicrobial activity using the paper disc agar diffusion method. Four components, named as compound 1, 2, 3, and 4 were found by the thin layer chromatography and ninhydrin color printing. The compounds with antimicrobial activity were detected and tracked by bioautography of Vibrio parahaemolyticus and triphenyltetrazolium chloride (TTC) coloration on the plate of thin layer chromatography. Two inhibition spots were found, corresponding to compound 1 and 2. The further study showed that the compound 1, 2, and 4 were stable at high temperature. Therefore, it was preliminarily deduced that the four compounds were all straight-chain lipopeptide produced at different phase of fermentation. |
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| Keywords: | Bacillus subtilis antimicrobial activity bioautography liner lipopeptides |
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