| 基于COI部分序列探讨南海鲳属鱼类系统进化与种群遗传结构 |
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| 引用本文： | 孙 鹏,尹 飞,施兆鸿,彭士明.基于COI部分序列探讨南海鲳属鱼类系统进化与种群遗传结构[J].海洋科学,2012,36(6):15-21. |
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| 作者姓名： | 孙 鹏 尹 飞 施兆鸿 彭士明 |
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| 作者单位： | 中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源及生态重点开放实验室,上海200090 |
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| 基金项目： | 国家科技支撑项目(2011BAD13B01); 上海市农委重点项目(沪农科攻字20074-1); 中央级公益性科研院所基本科研业务费项目(东2009M05, 东2009M08) |
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| 摘 要： | 利用线粒体细胞色素 c 氧化酶Ⅰ亚基(COI)部分序列研究了南海区鲳属鱼类(Pampus)银鲳(P. ar-genteus)、珍鲳(P. minor)和中国鲳(P. chinensis)的系统进化与种群遗传结构.通过 PCR 扩增与序列测定获得了长度为643 bp 的 COI 基因片段,其 A、T、G、C 碱基的平均含量分别为25.4%、33.6%、18.9%和22.1%, A+T 含量高于 G+C 含量.64条序列共定义了20种单倍型,包含152个变异位点,简约信息位点148个,单变异位点4个,产生169个点突变.结果表明,银鲳与中国鲳的遗传差异最小,银鲳与珍鲳的其次,而珍鲳与中国鲳的遗传差异最大,3种鲳属鱼类的遗传多样性均呈现较低的水平,应采取有效的保护措施,以避免其遗传多样性水平的进一步丧失.本研究结果为鲳属鱼类资源保护和合理开发利用提供必要的参考
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| 关 键 词： | 鲳属鱼类(Pampus) 细胞色素 c 氧化酶亚基Ⅰ(COI) 序列变异 系统进化 |
| 收稿时间： | 3/3/2011 12:00:00 AM |
| 修稿时间： | 2011/4/11 0:00:00 |
Phylogenetic relationships and population structure of Pampus in the South China Sea inferred from COI gene segments |
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| SUN Peng,YIN Fei,SHI Zhao-hong,PENG Shi-ming.Phylogenetic relationships and population structure of Pampus in the South China Sea inferred from COI gene segments[J].Marine Sciences,2012,36(6):15-21. |
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| Authors: | SUN Peng YIN Fei SHI Zhao-hong PENG Shi-ming |
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| Institution: | (Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China) |
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| Abstract: | Sequence variation and population genetic structure of Pampus argenteus,P.minor and P.chinensis in the South China Sea were evaluated using a 643 base pair fragment of mitochondrial cytochrome c oxidase subunit Ⅰ(COI) gene.The average contents of A,T,G and C were 25.4%,33.6%,18.9%,and 22.1%,respectively.The content of A+T was much higher.In 64 COI sequences,a total of 152 polymorphic sites(including 148 parsimony informative sites and 4 singleton variable sites) formed 169 mutations,and defined 20 distinct haplotypes.P.minor and P.chinensis had the largest genetic divergence,whereas P.argenteus and P.chinensis had the least genetic divergence.All three pampus populations presented low level of genetic diversity.Therefore,certain efficient measures should be made to avoid further decline of genetic diversity in those species.The present results provide basic information for the conservation and sustainable exploitation of Pampus species. |
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| Keywords: | Pampus mitochondrial cytochrome c oxidase subunitⅠ(COI) sequence variation phylogenetic evolution |
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