| 深海热液喷口金属硫化物中甲烷菌的多样性研究 |
| |
| 引用本文： | 王淑芳,陈 月,钱媛媛.深海热液喷口金属硫化物中甲烷菌的多样性研究[J].海洋科学,2012,36(6):31-38. |
| |
| 作者姓名： | 王淑芳 陈 月 钱媛媛 |
| |
| 作者单位： | 1. 淮海工学院海洋学院,江苏连云港222005
2. 淮海工学院江苏省海洋生物技术重点建设实验室,江苏连云港222005 |
| |
| 基金项目： | 淮海工学院引进人才基金项目(KQ09021) |
| |
| 摘 要： | 对两个来自太平洋洋脊胡安·德富卡的两个热液烟囱的金属硫化物4136-2和4148-B1进行甲烷菌甲基辅酶 M 还原酶的编码基因 mcrA 的序列扩增,构建克隆文库并进行分子进化分析.结果表明在这个甲烷富集的热液喷口周围含有丰富的甲烷产生菌,没有任何甲烷氧化菌存在.两个硫化物样品的甲烷产生菌种类完全不同.在4136-2硫化物中的甲烷产生菌都与热液口的高温环境有关系,主要属于甲烷球菌目的甲烷暖球菌(Methanocaldococcus),少部分属于甲烷火菌目甲烷嗜高热菌属的坎氏甲烷嗜高热菌(Methanopyrus kandleri).与这两个属的可分离菌株的氨基酸同源性为89%~97%,核苷酸同源性高达92%~100%.4148-B1硫化物中发现的一类疑似甲基辅酶 M 还原酶的编码序列,它们与已知的甲烷菌 mcrA 序列核苷酸同源性为69%~72%,氨基酸同源性仅为43%~47%.这可能是由于4148-B1来自于正在喷发的超高温热液喷口相关.由于与已发表的甲烷菌克隆子或菌株同源性较低,有可能是热液口特有的以前未发现的甲烷菌
|
| 关 键 词： | 胡安·德富卡 金属硫化物 甲烷产生菌 mcrA |
| 收稿时间： | 2011/6/25 0:00:00 |
| 修稿时间： | 2011/12/11 0:00:00 |
Studies on the biodiversity of methanogens from hydrothermal vent chimney |
| |
| WANG Shu-fang,CHEN Yue,QIAN Yuan-yuan.Studies on the biodiversity of methanogens from hydrothermal vent chimney[J].Marine Sciences,2012,36(6):31-38. |
| |
| Authors: | WANG Shu-fang CHEN Yue QIAN Yuan-yuan |
| |
| Institution: | 1(1.Huaihai Institute Technology,Oceanography Institute,Lianyungang 222005,China;2.Key Laboratory of Marine Biotechnology in Jiangsu Province,Huaihai Institute of Technology,Lianyungang 222005,China) |
| |
| Abstract: | The chimney sulfides (named 4136-2 and 4148-B1) obtained from Juan de Fuca Ridge were used as samples, aiming at mcrA gene amplification, clone library construction and phylogenetic tree analysis. The results showed that there were only CH4 production microbes but no CH4 oxidation microbes in the samples and the species in the two sulfides were different. The methanogen from 4136-2 sulfide rock were linked to a high temperature environment. These methanogen belong to Methanocaldococcus and Methanopyrus kandleri. The similarities of protein sequences between 4136-2 clones and isolated Methanocaldococcus and Methanopyrus kandleri strains were from 89% to 97%, and The similarities of mucleotide sequences between 4136-2 clones and isolated Methanocaldococcus and Methanopyrus kandleri strains were from 92% to 100%. The mcrA sequences from 4148-B1 sulfide rock had low identities with sequences deposited in GenBank. The similarities of mucleotide sequences identities of 4148-B1 clones and published mcrA sequences were form 69% to 72%, and the similarities of protein sequences were form 43% to 37%. This also linked to its super high temperature environment and may suggest there were un-discovered methanogen living in the vent. |
| |
| Keywords: | juan de fuca (JDF) sulfide methogen mcrA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《海洋科学》浏览原始摘要信息 |
| 点击此处可从《海洋科学》下载免费的PDF全文 |
|
