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海蒿子多糖DEI、DEII组分的分离纯化及单糖组成分析
引用本文:赵 宇,李俊卿,张立新,陈 悦,李志萍,陆懋荪,尹佩玉.海蒿子多糖DEI、DEII组分的分离纯化及单糖组成分析[J].海洋科学,2006,30(9):6-8.
作者姓名:赵 宇  李俊卿  张立新  陈 悦  李志萍  陆懋荪  尹佩玉
作者单位:青岛大学化工学院 山东青岛266071
基金项目:青岛市科技局资助项目(02-1-kchhh-42-2)
摘    要:海蒿子(SargassumconfusumAgardh)经热水抽提、乙醇沉淀、DEAE-SephadexA-25柱层析分离,得到多糖DEI、DEII,糖质量分数分别为52.40%,38.80%。经酸降解、薄层层析、气相色谱-质谱分析,证明其单糖组成均为岩藻糖、木糖、半乳糖、果糖、甘露糖、葡萄糖。其中岩藻糖质量分数最高(DEI:39.3%;DEII:35.2%)。

关 键 词:海蒿子(SargassumconfusumAgardh)  多糖  分离  纯化  单糖组成
收稿时间:2004/11/17 0:00:00
修稿时间:2005/6/23 0:00:00

The studies on the isolation, purification, and chemical composition analysis of polysaccharides DEI and DEII from Sargassum confusum
ZHAO Yu,LI Jun-qing,ZHANG Li-xin,CHEN Yue,LI Zhi-ping,LU Mao-sun,YIN Pei-yu.The studies on the isolation, purification, and chemical composition analysis of polysaccharides DEI and DEII from Sargassum confusum[J].Marine Sciences,2006,30(9):6-8.
Authors:ZHAO Yu  LI Jun-qing  ZHANG Li-xin  CHEN Yue  LI Zhi-ping  LU Mao-sun  YIN Pei-yu
Abstract:The characteristics of the polysaccharide DEI and polysaccharide DEII isolated from Sargassum pallidium through water extraction, ethanol precipitation and DEAE-Sephadex A-25 column chromatography sequentially were analyzed by TLC, gel electrophoresis and GC-MS. It was found that these polysaccharides were composed of fucose, xylose, galactose, fructose, mannose and glucose, among them fucose being the primarycomponent (39.3% in DEI and 35.2% in DEII).
Keywords:Sargassum confusum  polysaccharide  isolation  purification  chemical composition
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