| 海洋聚磷菌中核苷二磷酸激酶基因的克隆及序列分析 |
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| 引用本文： | 任世英,肖天.海洋聚磷菌中核苷二磷酸激酶基因的克隆及序列分析[J].海洋科学,2008,32(9):61-63. |
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| 作者姓名： | 任世英 肖天 |
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| 作者单位： | 1. 中国科学院,海洋研究所,山东,青岛,266071;中国科学院,研究生院,北京,100049
2. 中国科学院,海洋研究所,山东,青岛,266071 |
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| 基金项目： | 国家重点基础研究发展计划项目(2006CB400604); 国家自然科学基金资助项目(40376048) |
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| 摘 要： | 以海洋聚磷菌Halomonas YSR-3的总DNA为模板，用PCR法扩增核苷二磷酸激酶基因，将扩增片段克隆到pGM．T载体，转化Escherichia coli TOP10菌株，经蓝白斑筛选、菌落PCR得到阳性克隆，测序后对序列进行Blast比对分析。得到的基因序列长度为420bp，翻译后的序列与Loktanella vestfoldensis SKA53，Jannaschia sp．CCS1，Roseobacter sp．CCS2的核苷二磷酸激酶蛋白序列相似性分别为89％，86％，85％。
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| 关 键 词： | 盐单胞菌属 聚磷菌 核苷二磷酸激酶（NDPK） |
| 收稿时间： | 2008/2/28 0:00:00 |
| 修稿时间： | 2008/5/10 0:00:00 |
Cloning and analyse of nucleoside diphosphate kinase gene sequence from marine polyphosphate-accumulating bacterium, Halomonas YSR-3 |
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| REN Shi-ying,XIAO Tian.Cloning and analyse of nucleoside diphosphate kinase gene sequence from marine polyphosphate-accumulating bacterium, Halomonas YSR-3[J].Marine Sciences,2008,32(9):61-63. |
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| Authors: | REN Shi-ying XIAO Tian |
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| Abstract: | Nucleoside diphosphate kinase(NDPK) gene was amplified from marine polyphosphate-accumula- ting bacterium Halomonas YSR-3 by PCR and molecular clone techniques and inserted into a pGM-T vector.The recombinant plasmid was transformed into an Escherichia coli TOP10 strain.The positive transformants with NDPK gene were obtained by blue-white selection and clony PCR method,then the positive ones were sequenced. The sequenced fragment concluded 420 pairs of bases and was analyzed by NCBI database.The similaritie... |
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| Keywords: | Halomonas polyphosphate-accumulating bacterium nucleoside diphosphate kinase(NDPK) |
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